This recorded lecture introduces students to the quantification of "brightness," ways to quantify and compare bioluminescent and fluorescent proteins, and the factors that influence Förster …
The NeuroNex Technology Hub and NeuroNex Bioluminescent Constructs Database are supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award …
BioLuminescent-OptoGenetic molecules for driving and imaging neural activity have a number of advantages in vivo. In this approach, genetically expressed luciferases bind with injected luciferins …
As bioluminescence becomes increasingly significant to imaging and manipulating neurons in the brain, there presents a need for a database that can concisely present bioluminescent …
We developed a bioluminescent Ca2+ indicator, Lumicampsin (LMC) that employs a split, mutated Gaussia luciferase (sbGluc) with a CaM-M13 calcium sensing moiety introduced between the …
This double-sided placemat contains activities for students to complete as they learn about the creatures that produce bioluminescence and the differences between bioluminescence, fluorescence, and …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
In BioLuminescent driven OptoGenetics (BL-OG) a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. When light emitter …
This recorded lecture introduces undergraduate students to the advantages of bioluminescence as an optical reporter of a chemical reaction and its use with optogenetics (BL-OG) …
Bioluminescent sensors of intracellular calcium offer a number of advantages in vitro and in vivo. Calcium-sensing luciferases can be further tethered to specific voltage-gated Ca …
In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, …
Categories serve to reduce complexity and help us to navigate our social environment. At the same time, they can trap individuals in perceived or real …
The Bioluminescence Hub, a National Science Foundation NeuroNex Technology Hub, is dedicated both to the development of novel bioluminescent and optogenetic tools and to the …
This recorded lecture introduces undergraduate students to calcium as a ubiquitous second messenger and a proxy for cellular activity. Students learn why calcium is unique, …
Fluorescence-based optogenetic tools are powerful methods for dissecting cell-type specific circuit dynamics in the nervous system. These tools, however, rely on external, generic modification of …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
Interluminescence is a method for selective modulation of synaptic communication between genetically defined partners. Bioluminescent light from a pre-synaptic released luciferase activates a post-synaptic localized …
Using optogenetics, we can manipulate neuronal activity using light. Due to the young age of the animals used in this study, a non-invasive light source …
The malformation of neuronal circuitry in the developing neocortex leads to a variety of psychiatric disorders later in the organism’s life. By investigating alterations in …
This digital poster created for the 2021 BRAIN Initiative Investigators Meeting provides an overview of the advantages of bioluminescence for neuroscience research. Our BioLuminescent-OptoGenetics (BL-OG) …
Patterns of neuronal activity during early development are believed to guide the assembly of neural circuits. Thus, alterations of neuronal activity in this time window …
This recorded lecture introduces undergraduate students to the chemistry of bioluminescence and fluorescence. The lecture explains how new fluorescent proteins are discovered, whether in nature …
This recorded lecture introduces undergraduate students to genetic engineering, the creation of molecular constructs, laboratory techniques for in vitro study and viral transduction for in …
List of positions anterior-posterior positions relative to Bregma, as well as depth relative to skull mark and distance left to advance the electrode (along the …
This activity is designed for children ages 8-12 and will introduce them to the basics of bioluminescence and bioluminescent fungi. Learners will have the opportunity …
Using mRNA-Seq and de novo transcriptome assembly, we identified, cloned and characterized nine previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related …
Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal …
In BioLuminescent OptoGenetics, a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. Here, we leveraged this coupling …
In BioLuminescent OptoGenetics (BL-OG), the light sensing opsin, such as a channelrhodopsin or a pump, is activated by biological light emitted from a luciferase that …
This recorded interview is the sixth in our Interviews with Experts series and features Elena Goun, PhD, Associate Professor of Chemistry at the University of …
This recorded interview is the first in our Interviews with Experts series and features Henry Colecraft, PhD, the John C. Dalton Professor of Physiology and …
This recorded interview is the fourth in our Interviews with Experts series and features Jennifer Prescher, PhD, Professor of Chemistry, Molecular Biology and Biochemistry and …
This recorded interview is the fifth in our Interviews with Experts series and features Peter Hegemann, PhD, Professor and Head of the Department for Biophysics …
This recorded interview is the third in our Interviews with Experts series and features Stefan Herlitze, PhD, Neuroscientist and Faculty of Biology and Biotechnology at …
This recorded interview is the second in our Interviews with Experts series and features Steven Haddock, PhD, Senior Scientist/Marine Biologist at the Monterey Bay Aquarium …
This recorded lecture introduces undergraduate students to the behavior and rapid attenuation of light when it interacts with the ocean and to some of the …
This activity is a resource for parents, guardians, or educators of children ages 10-13 to introduce learners to the basics of luminescence while also giving …
Over recent years miniature microscopes have become a key tool for in vivo free behavioral studies. This technology offers the ability to measure network dynamics …
Bioluminescent optogenetics (BL-OG) uses light emitted from a luciferase in the presence of its luciferin to activate light sensing molecules, including channelrhodopsins and light-sensitive transcription …
This is a movie showing bioluminescence of the Choroid Plexus in a transgenic mouse expressing the LMO3 molecule. Water-Soluble Coelenterazine (CTZ) by Nanolight was injected …
This is a movie showing bioluminescence in left primary somatosensory cortex in a mouse expressing the LMO3 molecule. Water-Soluble Coelenterazine (CTZ) by Nanolight was injected …
This is a movie showing bioluminescence in left primary somatosensory cortex in a mouse expressing the LMO3 molecule. Water-Soluble Coelenterazine (CTZ) by Nanolight was injected …
This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in …
This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in …
Using neuroscientists' tools of the trade one can image in independent animals either the brain or the spinal cord. Yet understanding many biological processes such …
State-of-the-art imaging tools rely on a restrained preparation, limiting natural behavior while measuring cellular activity via fluorescence (FL). Recently, advances in wearable microscopy expanded the …
Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal …
Bioluminescent Optogenetics (BL-OG) is a novel approach for controlling neuronal activity in which light-sensing opsins are controlled by “biological light.” Luciferases, enzymes that produce bioluminescent …
To understand how groups of neurons participate in neural circuits underlying behavior, technologies are needed for observing and controlling the ensembles employed during behavior across …
A non-invasive and highly specific approach to neuronal stimulation is bioluminescent-optogenetics, where genetically expressed light emitting luciferases are tethered to light sensitive channelrhodopsins (luminopsins, LMO); …
The amount and pattern of neuronal activity present during the early developmental period is believed to guide structural and functional properties of neural circuit assembly. …
The amount and pattern of neuronal activity present during the early developmental period is believed to guide structural and functional properties of neural circuit assembly. …
A wealth of new tools can directly control output of specific neurons on fast (e.g., optogenetic) or sustained (e.g., chemogenetic) time scales. In contrast, almost …
Almost 30 years after identifying the genetic mutation underlying Huntington’s disease (HD), treatments remain limited to managing late-stage symptoms of motoric, psychiatric, and cognitive deficits. …
Bioluminescent molecules for imaging and modifying neural activity are increasingly becoming viable tools for in vivo use and have the potential to overcome many of …
Coelenterazine (CTZ) is the substrate of many luciferases and photoproteins critical for native and applied bioluminescence. CTZ has a long track-record and is welltolerated across …
As an alternative to fluorescent imaging, we are developing calcium-dependent, genetically-encoded molecules using bioluminescent probes. Bioluminescence is chemically generated light that occurs when a photon-containing …
Bioluminescent optogenetics (BL-OG) can be utilized for trans-synaptic neuronal communication by expressing the light emitting luciferase in the presynaptic and the light sensing opsin in …
Biological light activation of optogenetic sensors across synaptic partners offers the potential to optogenetically dissect synaptic communication non-invasively. The genetically encoded light source, a luciferase, …
Bioluminescent optogenetics (BL-OG) can be utilized for trans-synaptic neuronal communication by expressing the light emitting luciferase in the presynaptic and the light sensing opsin in …
This activity is designed for children ages 8-11 and guides learners through the basics of fluorescence while also giving them the opportunity to practice some …
This activity is designed for children ages 7-15 and introduces learners to living dinoflagellates and the basics of bioluminescence. While completing the activity, students will …
