Categories serve to reduce complexity and help us to navigate our social environment. At the same time, they can trap individuals in perceived or real …
Chemogenetic modulation of neuronal populations allows for precise control over cells engineered to express an exogenous receptor that responds exclusively to specific molecules. Chemogenetic approaches …
The malformation of neuronal circuitry in the developing neocortex leads to a variety of psychiatric disorders later in the organism’s life. By investigating alterations in …
This digital poster created for the 2021 BRAIN Initiative Investigators Meeting provides an overview of the advantages of bioluminescence for neuroscience research. Our BioLuminescent-OptoGenetics (BL-OG) …
Patterns of neuronal activity during early development are believed to guide the assembly of neural circuits. Thus, alterations of neuronal activity in this time window …
Despite ever improving treatment methods, there still is no way to effectively restore function after spinal cord injury. A promising approach is to re-engage spinal …
In BioLuminescent OptoGenetics, a genetically encoded light source, a luciferase, activates a light-sensing optogenetic element, a channelrhodopsin or a pump. Here, we leveraged this coupling …
Bioluminescent optogenetics (BL-OG) uses light emitted from a luciferase in the presence of its luciferin to activate light sensing molecules, including channelrhodopsins and light-sensitive transcription …
State-of-the-art imaging tools rely on a restrained preparation, limiting natural behavior while measuring cellular activity via fluorescence (FL). Recently, advances in wearable microscopy expanded the …
Bioluminescent Optogenetics (BL-OG) is a novel approach for controlling neuronal activity in which light-sensing opsins are controlled by “biological light.” Luciferases, enzymes that produce bioluminescent …
To understand how groups of neurons participate in neural circuits underlying behavior, technologies are needed for observing and controlling the ensembles employed during behavior across …
A wealth of new tools can directly control output of specific neurons on fast (e.g., optogenetic) or sustained (e.g., chemogenetic) time scales. In contrast, almost …
Coelenterazine (CTZ) is the substrate of many luciferases and photoproteins critical for native and applied bioluminescence. CTZ has a long track-record and is welltolerated across …
Bioluminescent optogenetics (BL-OG) can be utilized for trans-synaptic neuronal communication by expressing the light emitting luciferase in the presynaptic and the light sensing opsin in …
Bioluminescent optogenetics (BL-OG) can be utilized for trans-synaptic neuronal communication by expressing the light emitting luciferase in the presynaptic and the light sensing opsin in …