- Title Information
- Title
- HAP2(GCS1) is a conserved sperm-expressed
gene required for fertilization
- Name:
Personal
- Name Part
- Frank, Aubrey C
- Role
- Role Term:
Text
- creator
- Origin Information
- Copyright Date
- 2010
- Physical Description
- Extent
- xvi, 178 p.
- digitalOrigin
- born digital
- Note
- Thesis (Ph.D. -- Brown University (2010)
- Name:
Personal
- Name Part
- Johnson, Mark
- Role
- Role Term:
Text
- Director
- Name:
Personal
- Name Part
- DeLong, Alison
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wessel, Gary
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Petti, Wolfgang
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Snell, William
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. BIOMED: Molecular
Biology, Cell Biology, and Biochemistry
- Role
- Role Term:
Text
- sponsor
- Genre (aat)
- theses
- Abstract
- In flowering plants, two sperm cells develop in
the pollen cytoplasm and are transported through floral tissues to an
ovule by a pollen tube, a highly polarized cellular extension. After
targeting an ovule, the pollen tube bursts, releasing two sperm that
fertilize the egg and central cell producing the embryo and endosperm
respectively. The mechanisms responsible for fusion of the two sperm
with female cells are unknown. This thesis presents the initial
characterization of the Arabidopsis HAP2 gene, demonstrating that it is
essential for fertilization and probably directly involved in a deeply
conserved gamete fusion mechanism. We also show that HAP2 is required
for pollen tube guidance. We searched for proteins that interact with
HAP2 through a yeast two-hybrid screen and identified proteins that
regulate disulfide bond formation, these data indicate several invariant
cysteine residues within the N-terminal region of HAP2 are likely
critical for its structure and function. We utilized the tightly
regulated sperm-specific expression pattern of HAP2 to disrupt sperm
development by expressing the Diphtheria toxin A subunit. By disrupting
sperm translation in this manner, single sperm-like cells (SSLCs) were
generated that preferentially fertilized the central cell. This finding
is contrary to the previously held idea that SSLCs like those created in
the cdc2 mutant have a default targeting to the egg. We were able to
ectopically express HAP2 in the pollen tube, but were unable to find
transformants that express HAP2 in the leaves when expressed from the
35S promoter. These experiments begin to elucidate the expression
pattern, proper folding, and function of HAP2 during fertilization.
- Subject
- Topic
- HAP2
- Subject
- Topic
- fertilization
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/923193")
- Topic
- Fertilization (Biology)
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/812564")
- Topic
- Arabidopsis thaliana
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1015841")
- Topic
- Membrane fusion
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20110926
- Language
- Language Term:
Code (ISO639-2B)
- eng
- Language Term:
Text
- English
- Identifier:
DOI
- 10.7301/Z0VT1Q96
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Type of Resource (primo)
- dissertations