<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:METS="http://www.loc.gov/METS/" ID="etd361" xsi:schemaLocation="http://www.loc.gov/mods/v3  http://www.loc.gov/standards/mods/v3/mods-3-2.xsd">
          <mods:titleInfo>
            <mods:title>HAP2(GCS1) is a conserved sperm-expressed 
gene required for fertilization </mods:title>
          </mods:titleInfo>
          <mods:name type="personal">
            <mods:namePart>Frank, Aubrey C</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">creator</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:originInfo>
            <mods:copyrightDate>2010</mods:copyrightDate>
          </mods:originInfo>
          <mods:physicalDescription>
            <mods:extent>xvi, 178 p.</mods:extent>
            <mods:digitalOrigin>born digital</mods:digitalOrigin>
          </mods:physicalDescription>
          <mods:note>Thesis (Ph.D. -- Brown University (2010)</mods:note>
          <mods:name type="personal">
            <mods:namePart>Johnson, Mark</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">Director</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:name type="personal">
            <mods:namePart>DeLong, Alison</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">Reader</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:name type="personal">
            <mods:namePart>Wessel, Gary</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">Reader</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:name type="personal">
            <mods:namePart>Petti, Wolfgang</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">Reader</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:name type="personal">
            <mods:namePart>Snell, William</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">Reader</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:name type="corporate">
            <mods:namePart>Brown University. BIOMED: Molecular 
Biology, Cell Biology, and Biochemistry</mods:namePart>
            <mods:role>
              <mods:roleTerm type="text">sponsor</mods:roleTerm>
            </mods:role>
          </mods:name>
          <mods:genre authority="aat">theses</mods:genre>
          <mods:abstract>	In flowering plants, two sperm cells develop in 
the pollen cytoplasm and are transported through floral tissues to an 
ovule by a pollen tube, a highly polarized cellular extension. After 
targeting an ovule, the pollen tube bursts, releasing two sperm that 
fertilize the egg and central cell producing the embryo and endosperm 
respectively.  The mechanisms responsible for fusion of the two sperm 
with female cells are unknown.  This thesis presents the initial 
characterization of the Arabidopsis HAP2 gene, demonstrating that it is 
essential for fertilization and probably directly involved in a deeply 
conserved gamete fusion mechanism. We also show that HAP2 is required 
for pollen tube guidance.  We searched for proteins that interact with 
HAP2 through a yeast two-hybrid screen and identified proteins that 
regulate disulfide bond formation, these data indicate several invariant
 cysteine residues within the N-terminal region of HAP2 are likely 
critical for its structure and function. We utilized the tightly 
regulated sperm-specific expression pattern of HAP2 to disrupt sperm 
development by expressing the Diphtheria toxin A subunit.  By disrupting 
sperm translation in this manner, single sperm-like cells (SSLCs) were 
generated that preferentially fertilized the central cell.  This finding 
is contrary to the previously held idea that SSLCs like those created in 
the cdc2 mutant have a default targeting to the egg.  We were able to 
ectopically express HAP2 in the pollen tube, but were unable to find 
transformants that express HAP2 in the leaves when expressed from the 
35S promoter.  These experiments begin to elucidate the expression 
pattern, proper folding, and function of HAP2 during fertilization.  
</mods:abstract>
          <mods:subject>
            <mods:topic>HAP2</mods:topic>
          </mods:subject>
          <mods:subject>
            <mods:topic>fertilization</mods:topic>
          </mods:subject>
          <mods:subject authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/923193"><mods:topic>Fertilization (Biology)</mods:topic></mods:subject><mods:subject authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/812564"><mods:topic>Arabidopsis thaliana</mods:topic></mods:subject><mods:subject authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/1015841"><mods:topic>Membrane fusion</mods:topic></mods:subject><mods:recordInfo>
            <mods:recordContentSource authority="marcorg">RPB</mods:recordContentSource>
            <mods:recordCreationDate encoding="iso8601">20110926</mods:recordCreationDate>
          </mods:recordInfo>
        <mods:language><mods:languageTerm type="code" authority="iso639-2b">eng</mods:languageTerm><mods:languageTerm type="text">English</mods:languageTerm></mods:language><mods:identifier type="doi">10.7301/Z0VT1Q96</mods:identifier><mods:accessCondition type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition><mods:accessCondition type="restriction on access">Collection is open for research.</mods:accessCondition><mods:typeOfResource authority="primo">dissertations</mods:typeOfResource></mods:mods>