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Year:: 2020
Contributor:: Gupta, Hersh (creator); Mincer, Joshua (thesis advisor); Istrail, Sorin (reader); Brown University. Center for Computational Molecular Biology (sponsor)
Genre:: theses
Subject:: Nucleotide sequence; Cancer; Computational biology
DOI: https://doi.org/10.26300/fqjk-t119

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Abstract:: Anesthesia, in particular opioids, may have significant clinical implications in cancer. This effect cannot be generalized across different cancer types, and this likely holds true across cancer subtypes as well. From unpublished retrospective clinical data, lung adenocarcinoma and triple negative breast cancer outcomes were shown to be negatively and positively impacted, respectively. Using retrospective genomic analysis and general knowledge of opioid mechanism of actions, bulk RNA sequencing and single cell RNA sequencing was analyzed to determine not only what cellular pathways are being impacted by opioid exposure, but also where in the tumor microenvironment this mechanism of action. Through this analysis, initial guesses to the cells of action indicate that the tumor in lung adenocarcinoma and immune cells in triple negative breast cancer are the likely site. This work hopes to inform future research at all levels of experiments: clinical, animal models, genomic, and computational.
Notes:: Senior thesis (AB)--Brown University, 2020; Concentration: Computational Biology

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Rights: In Copyright
Restrictions on Use: Collection is open for research.

Citation

Gupta, Hersh, "Exploration of possible molecular mechanisms of action of clinical effects of opioids in lung adenocarcinoma and triple negative breast cancer through RNA sequencing." (2020). Center for Computational Molecular Biology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/fqjk-t119

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Center for Computational Molecular Biology Theses and Dissertations

Theses and Dissertations for the Center for Computational Molecular Biology department.
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Exploration of possible molecular mechanisms of action of clinical effects of opioids in lung adenocarcinoma and triple negative breast cancer through RNA sequencing.