- Title Information
- Title
- Characterization of Macrophage Responses to Lung Microbiome Commensal Bacteria
- Abstract
- The human body is host to a variety of microbiomes, communities of microorganisms
known to affect human development and function. The lung microbiome, in contrast to the gut
microbiome, has been greatly understudied. Differences in lung microbiome diversity have been
correlated with the development of cancer, the pathogenesis of chronic obstructive pulmonary
disease (COPD), and are thought to contribute to the pathogenesis of asthma, pneumonia, and
influenza. The specific bacteria present in the lung have been identified by next-generation
sequencing analysis. However, to study the interaction of these bacteria with the cells of the lung,
we must be able to isolate and culture them.
Macrophages are phagocytic cells of the innate immune system whose functions range
from pro-inflammatory and anti-inflammatory responses to tissue repair. Tissue-resident
macrophages perform immune surveillance, respond to infection, and regulate inflammation in the
specific microenvironments of the body. In the lung, alveolar macrophages are by far the most
common immune cell in the steady state. It is unknown how alveolar macrophages interact with
the commensal bacteria of the lung microbiome, particularly how commensal bacteria do not
trigger constant immune activation and inflammation. Understanding the response of alveolar
macrophages to commensal bacteria from the lung microbiome could give insight as to why
changes in the lung microbiome can lead to disease states such as cancer and COPD.
This project sought to isolate the specific bacteria of the mouse lung microbiome, identify
the bacteria based on sequencing analysis, culture these bacteria into a library of commensal
bacteria, and then use this library to analyze the effects of the commensal bacteria upon
macrophages. Completing these steps allowed us to determine the effects these commensal
bacteria from the lung have upon the immune system.
- Name:
Personal
- Name Part
- Piatski, Marie E.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
- creator
- Name:
Personal
- Name Part
- Jamieson, Amanda
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/ths")
- thesis advisor
- Name:
Corporate
- Name Part
- Brown University. Molecular Microbiology and Immunology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2020
- Type of Resource
- text
- Physical Description
- digitalOrigin
- born digital
- Language
- Language Term:
Text (ISO639-2B)
(authorityURI="http://id.loc.gov/vocabulary/iso639-2.html", valueURI="http://id.loc.gov/vocabulary/iso639-2/eng")
- English
- Note:
thesis
- Senior thesis (ScB)--Brown University, 2020
- Note
(displayLabel="Concentration")
- Biochemistry & Molecular Biology
- Genre (aat)
- theses
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00825152")
- Topic
- Bacteria
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01005265")
- Topic
- Macrophages
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01003744")
- Topic
- Lungs
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- All rights reserved. Collection is open for research.
- Identifier:
DOI
- 10.26300/5215-ee84