- Title Information
- Title
- A Scalable 3D Printed Microfluidic Device for Rapid Preclinical Assays of Cancer Samples
- Type of Resource
- text
- Name:
Personal
- Name Part
- Markoski, Alex
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Wong, Ian
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Morgan, Jeffrey
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Reichner, Jonathan
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Borenstein, Jeffrey
- Role
- Role Term:
Text
- Advisor
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2020
- Physical Description
- Extent
- xi, 56 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2020
- Genre (aat)
- theses
- Abstract
- Immunotherapies such as immune checkpoint inhibitors (ICI) show great promise as more efficacious treatments for various cancers compared to current methods like chemotherapy. However, preclinical evaluation of immunotherapies typically relies on mouse models that have questionable relevance to human responses, or on in vitro culture methods that suffer from small sample sizes, limited cell viability, a lack of physiologically relevant stimuli such as flow, and an absence of tumor-immune cell interactions. To address these limitations with current models, a scalable 3D printed microfluidic device capable of capturing multicellular tumor spheroids and perfusing them to extend sample viability for long term testing was developed. Finite element modeling was used to optimize flow conditions and capture efficiency. Tumor spheroids were used to validate extended sample viability over three days compared to standard static culture. The resulting device created was capable of being printed and ready for use within an hour and a half with novel integrated connectors for scalability and ease of use. An array of 3D printed micro posts was able to capture and hold both tumor spheroids and tumor fragments in line with flow for perfusion over time. Ultimately, these microfluidic devices will enable researchers and clinicians alike to test various immunotherapies in a scalable, rapid, and in vivo relevant manner.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01747062")
- Topic
- Microfluidic devices
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00845386")
- Topic
- Cancer--Immunotherapy
- Subject
- Topic
- tumor spheroids
- Subject
- Topic
- 3D Printed
- Subject
- Topic
- preclinical assay
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20200720
- Identifier:
DOI
- 10.26300/hxqj-x070
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- All rights reserved. Collection is open to the Brown community for research.