- Title Information
- Title
- The Genetic Architecture of Flight and Climbing Performance in Drosophila Melanogaster
- Name:
Personal
- Name Part
- Spierer, Adam Noah
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Rand, David
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Ramachandran, Sohini
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Roberts, Thomas
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Tatar, Marc
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Ecological and Evolutionary Biology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2020
- Physical Description
- Extent
- xxvi, 178 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Ph. D.)--Brown University, 2020
- Genre (aat)
- theses
- Abstract
- A central focus of genetics seeks to understand how genotype contributes to phenotype. Simple, single gene traits are generally driven by alleles with additive or dominant effects. However, most traits are polygenic, or complex, and are the result of several genes working together. In addition to additive and dominant effects, pairwise (epistatic) and environmental effects also contribute to the assembly of genetic factors (genetic architecture) that modulate phenotypic variation. These factors can be further complicated by higher-order effects, like genotype x environment interactions, genotype x genotype interactions, and genotype x genotype x environment interactions. Testing the contribution of every possible combination affecting a trait’s genetic architecture is not feasible. However, we can take various approaches to look more broadly at the genotype-phenotype map with Genome Wide Association Studies (GWAS), or assess higher-order effects, via mitochondrial-nuclear (mito-nuclear) introgressions. Using a genetically tractable Drosophila model, we sought to uncover the genetic architecture of adult insect locomotion across flight and climbing behaviors. Divided across four chapters, we survey the genetic architecture of powered flight performance in two chapters using the 197 Drosophila Genetic Reference Panel (DGRP) lines where we identified and validated genetic modifiers associated with flight (i) ability and (ii) variability. In the other two chapters, (iii) we evaluated higher-order genotype x genotype x environment effects between mito-nuclear introgressions and their climbing performance following an exercise conditioning program. (iv) We also created a novel image analysis pipeline to quantify group climbing performance (FreeClimber), which we tested on a panel of exercise conditioned mito-nuclear introgression lines that contained two previously untested lines. Through this work, we expand the growing body of knowledge surrounding the genetic modifiers affecting complex traits and we contribute freely available, open source tools to facilitate repeatable data collection and biologically meaningful analyses.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00917991")
- Topic
- Exercise
- Subject
- Topic
- Genome Wide Association
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00898394")
- Topic
- Drosophila--Genetics
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01085068")
- Topic
- Quantitative genetics
- Subject
- Topic
- FreeClimber
- Subject
- Topic
- PEGASUS_flies
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00974141")
- Topic
- Insects--Locomotion
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00974115")
- Topic
- Insects--Flight
- Subject
- Topic
- Mitonuclear Interactions
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20200720
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Identifier:
DOI
- 10.26300/cck2-c765
- Type of Resource (primo)
- dissertations