<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-7.xsd">
<mods:titleInfo>
<mods:title>DCLK1 Inhibits Vesicular Trafficking in Non-Neuronal Cells</mods:title>
</mods:titleInfo>
<mods:abstract>Normal brain development, involving neuronal migration, polarity and differentiation, requires
synaptic trafficking of essential cargo. These cargo are primarily transported on microtubules
(MTs), which play a key role in neuronal morphology. Mutations in MT-related proteins were
previously found to be associated with epilepsy.
Specifically, Dcx and Dclk1 (doublecortin family proteins) were found to regulate Kif1a (a kinesin
motor protein) mediated transport and localization in neurons (Liu et. al 2012). However, it is
unknown whether Dclk1 alone is sufficient for transport.Vamp2, a vesicle membrane protein
carried by Kif1a, has been found to be transported by other kinesins, including Kif53 and Kif23.6
In particular, these kinesins are expressed significantly more in conventional non-neuronal HEK
and HeLa cells as opposed to Kif1a7. It is possible that Dclk1 plays a different role in trafficking
when Kif1a is not the primary transporter of Vamp2-containing vesicles.
Our experiments show that Dclk1 inhibits mobile vesicle movement in two cell culture models
expressing low levels of Kif1a, indicating a potential role for Dclk1 in mediating other modes of
vesicle trafficking.</mods:abstract>
<mods:name type="personal">
<mods:namePart>Lee, Jenny</mods:namePart>
<mods:role>
<mods:roleTerm type="text">creator</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>McCarthy-Sinclair, Brendan</mods:namePart>
<mods:role>
<mods:roleTerm type="text">creator</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Papendorf, Carin</mods:namePart>
<mods:role>
<mods:roleTerm type="text">creator</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Liu, Judy S.</mods:namePart>
<mods:role>
<mods:roleTerm type="text">creator</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Dr. Judy S. Liu</mods:namePart>
<mods:role>
<mods:roleTerm type="text">advisor</mods:roleTerm>
</mods:role>
<mods:affiliation>Brown University. Department of Molecular Biology, Cellular Biology, and Biochemistry</mods:affiliation>
</mods:name>
<mods:name type="corporate">
<mods:namePart>Brown University. Karen T. Romer Undergraduate Teaching and Research Awards</mods:namePart>
<mods:role>
<mods:roleTerm type="text">research program</mods:roleTerm>
</mods:role>
</mods:name>
<mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/01024734">
<mods:topic>Molecular biology</mods:topic>
</mods:subject>
<mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/00914183">
<mods:topic>Epilepsy</mods:topic>
</mods:subject>
<mods:language>
<mods:languageTerm type="text" authority="iso639-2b">English</mods:languageTerm>
</mods:language>
<mods:typeOfResource>still image</mods:typeOfResource>
<mods:genre authority="aat">posters</mods:genre>
<mods:originInfo>
<mods:place>
<mods:placeTerm type="code" authority="marccountry">riu</mods:placeTerm>
</mods:place>
<mods:place>
<mods:placeTerm type="text">Providence, RI</mods:placeTerm>
</mods:place>
<mods:publisher>Brown University</mods:publisher>
<mods:dateCreated keyDate="yes" encoding="w3cdtf">2019
</mods:dateCreated>
</mods:originInfo>
<mods:physicalDescription>
<mods:extent>1 poster</mods:extent>
<mods:digitalOrigin>born digital</mods:digitalOrigin>
</mods:physicalDescription>
<mods:identifier type="doi">10.26300/jf7r-d707</mods:identifier>
<mods:accessCondition type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition>
</mods:mods>