- Title Information
- Title
- Investigations of Complex Guidance Cues for Nerve Regeneration Using Novel In Vitro Platforms
- Name:
Personal
- Name Part
- Kofron, Celinda M.
- Role
- Role Term:
Text
- creator
- Origin Information
- Copyright Date
(keyDate="yes", encoding="w3cdtf")
- 2009
- Physical Description
- Extent
- xvi, 223 p.
- digitalOrigin
- born digital
- Note
- Thesis (Ph.D.) -- Brown University (2009)
- Name:
Personal
- Name Part
- Hoffman-Kim, Diane
- Role
- Role Term:
Text
- director
- Name:
Personal
- Name Part
- Morgan, Jeffrey
- Role
- Role Term:
Text
- reader
- Name:
Personal
- Name Part
- Palmore, Tayhas
- Role
- Role Term:
Text
- reader
- Name:
Personal
- Name Part
- Webster, Thomas
- Role
- Role Term:
Text
- reader
- Name:
Personal
- Name Part
- Thompson, Deanna
- Role
- Role Term:
Text
- reader
- Name:
Corporate
- Name Part
- Brown University. Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Genre (aat)
- theses
- Abstract
- Neurons have highly developed pathfinding abilities, but in any part of the nervous system, during development or after the challenge of injury, cues provided by the local
environment are required to trigger growth, direct paths, and guide connection or reconnection with appropriate targets. Study of these cues with in vitro platforms that are closer to in vivo
environments in architecture and complexity provide insights into neuronal integration of cues to inform strategies for nerve regeneration. Oriented tissue structures guide neurons in vitro and
in vivo. Biomimetic replica materials presenting aligned glial or non-glial cellular topographies, which isolate physical cues from biochemical cues, were all shown to guide neuronal growth
despite distinct topographies and anisotropies presented by different cells. Application of statistical design and analysis methods improved efficiency and utility in determining optimal
conditions for generating cellular monolayers for replication. Neuronal growth on materials presenting sharper, higher edges was more directed than growth on biomimetic materials suggesting that
the topography of anisotropic tissues are sufficient for guidance, but may not be optimal for precise control of directed neuronal growth. These biomimetic replica materials were also
investigated in combination with permissive and inhibitory biochemical cues to identify collaborative and hierarchical effects of directive cues. Neurons exhibited directional preference for
biochemical cues over cellular topographies in the limited range of protein concentrations and patterns tested. Comparison of the relative strength of cues when presented individually did not
effectively predict the hierarchy or accord of these cues when presented together. Neuronal preference for biochemical tracks was also examined in a three dimensional (3D) model. In two
dimensions, neurons align on permissive protein tracks and between inhibitory tracks. Neurons also aligned to permissive tracks at the surface of a 3D platform, but extended away from the
surface in 3D when an inhibitory molecule was presented. These findings increase the understanding of neuronal response to structurally complex environments and provide insights into the net
response of neurons to multiple cooperating or competing cues.
- Subject (Local)
- Topic
- Schwann cell
- Subject (Local)
- Topic
- design of experiments
- Subject (Local)
- Topic
- response surface methodology
- Subject (Local)
- Topic
- axon guidance
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1036436")
- Topic
- Neurons
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/819380")
- Topic
- Astrocytes
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1893632")
- Topic
- Endothelial cells
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/918404")
- Topic
- Experimental design
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1062032")
- Topic
- Photolithography
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20091218
- Language
- Language Term:
Code (ISO639-2B)
- eng
- Language Term:
Text
- English
- Identifier:
DOI
- 10.7301/Z0JQ0Z9W
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Type of Resource (primo)
- dissertations