Title Information
Title
Active metal oxides and polymer hybrids as biomaterials
Name: Personal
Name Part
Jarrell, John D.
Role
Role Term: Text
creator
Origin Information
Copyright Date (keyDate="yes", encoding="w3cdtf")
2008
Physical Description
Extent
xix, 212 p.
digitalOrigin
born digital
Note
Thesis (Ph.D.) -- Brown University (2008)
Name: Personal
Name Part
Morgan, Jeffrey
Role
Role Term: Text
director
Name: Personal
Name Part
Mathiowitz, Edith
Role
Role Term: Text
reader
Name: Personal
Name Part
Tripathi, Anubhav
Role
Role Term: Text
reader
Name: Personal
Name Part
Hoffman-Kim, Diane
Role
Role Term: Text
reader
Name: Personal
Name Part
Lee, William
Role
Role Term: Text
reader
Name: Corporate
Name Part
Brown University. Biomedical Engineering
Role
Role Term: Text
sponsor
Genre (aat)
theses
Abstract
Bone anchored prosthetic attachments, like other percutaneous devices, suffer from poor soft tissue integration, seen as chronic inflammation, infection, epithelial downgrowth and regression. We looked at the use of metal oxides as bioactive agents that elicit different bioresponses, ranging from cell attachment, tissue integration and reduction of inflammation to modulation of cell proliferation, morphology and microbe killing. This study presents a novel method for creating titanium oxide and polydimethylsiloxane (PDMS) hybrid coated microplates for high throughput biological, bacterial and photocatalytic screening that overcomes several limitations of using bulk metal samples. Titanium oxide coatings were doped with silver, zinc, vanadium, aluminum, calcium and phosphorous, while PDMS was doped with titanium, vanadium and silver and subjected to hydrothermal heat treatment to determine the influence of chemistry and crystallinity on the viability, proliferation and adhesion of human fibroblasts, keratinocytes and Hela cells. Also explored was the influence of Ag and Zn doping on E. coli proliferation. We determined how titanium concentration in hybrids and silver doping influenced the photocatalytic degradation of methylene blue by coatings. A combined sub/percutaneous, polyurethane device was developed and implanted into the backs of CD hairless rats to investigate how optimized coatings influenced soft tissue integration in vivo. We demonstrate that the bioresponse of cells to coatings is controlled by elemental doping (V & Ag) and that planktonic bacterial growth was greatly reduced or stopped by Ag, but not Zn doping. Hydrothermal heat treatments (65 °C and 121 °C) did not greatly influence cellular bioresponse to coatings. We discovered a range of temperature resistant (up to 400 °C), solid state dispersions with enhanced ability to block full spectrum photon transmission and degrade methylene using medical x-rays, UV, visible and infrared photons. We show that silver doping improved the photoactivity of oxide coatings, but hindered activity of a specific hybrid. Doped titanium oxide and polymer hybrid coatings have potential for improving soft tissue integration of medical implants and wound healing by modulating cell proliferation, attachment, inflammation and providing controlled delivery of bioactive and antimicrobial compounds and photon induced electro-chemical activity.
Subject (Local)
Topic
Titanium oxide
Subject (Local)
Topic
Hela
Subject (Local)
Topic
Crystallinity
Subject (Local)
Topic
Surface modification
Subject (Local)
Topic
Cell viability
Subject (Local)
Topic
Sol-gel techniques
Subject (Local)
Topic
Metal ion release
Subject (Local)
Topic
Bioactivity
Subject (Local)
Topic
Phosphorous
Subject (Local)
Topic
Percutaneous implants
Subject (Local)
Topic
Bone anchored prosthetics
Subject (Local)
Topic
Small animal models
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1151576")
Topic
Titanium dioxide
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/923669")
Topic
Fibroblasts
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/986853")
Topic
Keratinocytes
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/850224")
Topic
Cell proliferation
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/850164")
Topic
Cell adhesion
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1763742")
Topic
Polydimethylsiloxane
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1118837")
Topic
Silver
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1164010")
Topic
Vanadium
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/806262")
Topic
Aluminum
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/844035")
Topic
Calcium
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1061449")
Topic
Phosphorus
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1181480")
Topic
Wound healing
Record Information
Record Content Source (marcorg)
RPB
Record Creation Date (encoding="iso8601")
20091218
Language
Language Term: Code (ISO639-2B)
eng
Language Term: Text
English
Identifier: DOI
10.7301/Z08G8J01
Access Condition: rights statement (href="http://rightsstatements.org/vocab/InC/1.0/")
In Copyright
Access Condition: restriction on access
Collection is open for research.
Type of Resource (primo)
dissertations