<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:METS="http://www.loc.gov/METS/" xmlns:fits="http://hul.harvard.edu/ois/xml/ns/fits/fits_output" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:IR="http://dl.lib.brown.edu/md/irdata" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:rights="http://cosimo.stanford.edu/sdr/metsrights/" ID="etd66">
     <mods:titleInfo>
      <mods:title>Axon guidance by molecular and topographical cues</mods:title>
     </mods:titleInfo>
     <mods:name type="personal">
      <mods:namePart>Li, Grace N.</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">creator</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:originInfo>
      <mods:copyrightDate keyDate="yes" encoding="w3cdtf">2008</mods:copyrightDate>
     </mods:originInfo>
     <mods:physicalDescription>
      <mods:extent>xxvi, 220 p.</mods:extent>
      <mods:digitalOrigin>born digital</mods:digitalOrigin>
     </mods:physicalDescription>
     <mods:note>Thesis (Ph.D.) -- Brown University (2008)</mods:note>
     <mods:name type="personal">
      <mods:namePart>Hoffman-Kim, Diane</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">director</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:name type="personal">
      <mods:namePart>Lysaght, Michael</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">reader</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:name type="personal">
      <mods:namePart>Morgan, Jeffrey</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">reader</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:name type="personal">
      <mods:namePart>Tripathi, Anubhav</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">reader</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:name type="personal">
      <mods:namePart>Bellamkonda, Ravi</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">reader</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:name type="corporate">
      <mods:namePart>Brown University. Biomedical Engineering</mods:namePart>
      <mods:role>
       <mods:roleTerm type="text">sponsor</mods:roleTerm>
      </mods:role>
     </mods:name>
     <mods:genre authority="aat">theses</mods:genre>
     <mods:abstract>Traumatic injury to the spinal cord often results in irreversible loss of function as the central nervous system fails to spontaneously regenerate. The application of molecular and
      topographical guidance cues has been shown to direct neurite growth. Biomaterial platforms incorporating chemotropic, topographic and physical cues allow the investigation of how neurons
      integrate complex guidance information to make growth decisions. Microfluidic and micropatterning methods described provide a valuable tool towards fabricating these in vitro platforms. Adsorbed
      laminin (LN) and chondroitin sulfate proteoglycan (CSPG) gradients demonstrate that neurites extend toward regions of lower CSPG and higher LN concentrations. When the two contrasting cues were
      presented together, neurites responded differently depending on the directions of the gradients. The methods of circular statistical analysis that have been developed allow more accurate
      evaluation of neurite directional data. A three-dimensional approach to studying guidance cues was applied to biological matrices to identify the mechanisms involved in the regulation of neurite
      outgrowth. By comparing gene expression of neuroblastoma cells cultured in collagen or Matrigel, genomic profiles were screened using DNA microarrays. Selected genes were further evaluated by
      reversetranscriptase polymerase chain reaction. Complex cell?material interactions were observed in 3D, where the dimension of the culture material influenced gene expression and cell spreading,
      while the structural and mechanical properties of the culture material influenced gene expression and neurite outgrowth. Growth and morphology of Schwann cell (SC) cultures on microgrooved
      topography were also studied. The dynamics of SC bridging was observed and analyzed under timelapse microscopy. Highly motile SCs were observed and trajectories included a climbing phase and a
      stabilization phase. Combining molecular and topographical guidance information by selectively coating LN on microgrooves showed that protein coating of walls and grooves was essential for SC
      bridging. The combination of LN and topography yielded a synergistic effect between the guidance cues. The platforms and studies described herein allow us to investigate interactions between
      neurons, glia and their microenvironment and to understand the molecular basis for these interactions that regulate neurite outgrowth.</mods:abstract>
     <mods:subject authority="local">
      <mods:topic>neurite outgrowth</mods:topic>
     </mods:subject>
     <mods:subject authority="local">
      <mods:topic>micropatterning</mods:topic>
     </mods:subject>
     <mods:subject authority="local">
      <mods:topic>molecular gradients</mods:topic>
     </mods:subject>
     <mods:subject authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/918995"><mods:topic>Extracellular matrix</mods:topic></mods:subject><mods:recordInfo>
      <mods:recordContentSource authority="marcorg">RPB</mods:recordContentSource>
      <mods:recordCreationDate encoding="iso8601">20091218</mods:recordCreationDate>
     </mods:recordInfo>
    <mods:language><mods:languageTerm type="code" authority="iso639-2b">eng</mods:languageTerm><mods:languageTerm type="text">English</mods:languageTerm></mods:language><mods:identifier type="doi">10.7301/Z0KP80F3</mods:identifier><mods:accessCondition type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition><mods:accessCondition type="restriction on access">Collection is open for research.</mods:accessCondition><mods:typeOfResource authority="primo">dissertations</mods:typeOfResource></mods:mods>