Title Information
Title
Translational control and active transport of Vg1 mRNA in Xenopus laevis oocytes
Name: Personal
Name Part
Pratt, Catherine A
Role
Role Term: Text
creator
Origin Information
Copyright Date
2013
Physical Description
Extent
xv, 195 p.
digitalOrigin
born digital
Note
Thesis (Ph.D. -- Brown University (2013)
Name: Personal
Name Part
Mowry, Kimberly
Role
Role Term: Text
Director
Name: Personal
Name Part
Dahlberg, Albert
Role
Role Term: Text
Reader
Name: Personal
Name Part
Laney, Jeffrey
Role
Role Term: Text
Reader
Name: Personal
Name Part
Reenan, Robert
Role
Role Term: Text
Reader
Name: Personal
Name Part
Vasudevan, Shobha
Role
Role Term: Text
Reader
Name: Corporate
Name Part
Brown University. BIOMED: Molecular Biology, Cell Biology, and Biochemistry
Role
Role Term: Text
sponsor
Type of Resource
text
Genre (aat)
theses
Abstract
mRNA localization is a powerful post-transcriptional mechanism of gene expression that allows for protein expression in distinct cellular locations. This process is exploited in a number of organisms and cell types, and has been particularly well studied in developmental contexts. One such model system is that of the Vg1 mRNA which is localized to the vegetal cortex of the Xenopus laevis oocyte. The primary focus of this dissertation is an investigation into how the Vg1 mRNA is translationally silenced during localization. The results of this investigation are presented in Chapter 2, and implicate the miRNA pathway, specifically Argonaute proteins and the let-7 family of miRNAs, in translational repression. In addition, known Xenopus RNA-binding proteins were investigated as potential translational repressors, and the results of these experiments are presented in the Appendix. In the process of this investigation an in vitro translation extract from Xenopus oocytes was developed, which is documented in Chapter 3. A secondary aim was to understand how Vg1 is localized along the microtubule cytoskeleton. Chapter 4 shows that the orientation of the microtubule cytoskeleton at the vegetal pole of the oocyte and the activity of kinesin motor proteins are critical for the transport of this mRNA. Together, these data represent novel insights into mRNA transport and translational control.
Subject
Topic
mRNA localization
Subject
Topic
translational repression
Subject
Topic
Vg1
Subject
Topic
miRNA
Subject
Topic
Argonaute
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1182017")
Topic
Xenopus laevis
Record Information
Record Content Source (marcorg)
RPB
Record Creation Date (encoding="iso8601")
20131217
Language
Language Term: Code (ISO639-2B)
eng
Language Term: Text
English
Identifier: DOI
10.7301/Z0639N1P