<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-4.xsd">
  <mods:titleInfo>
    <mods:title>Creating a CLAMP null using the CRISPR/Cas system</mods:title>
  </mods:titleInfo>
  <mods:abstract>In all organisms, gene regulation is crucial for proper cell function and development. Dosage compensation is one such mechanism of gene regulation. In Drosophila melanogaster, dosage compensation is mediated by the MSL (Male Specific Lethal) protein complex. The MSL complex allows for the two-fold up-regulation of the X chromosome in males (XY), in order to equalize the disparities in amount of genetic information with females (XX). Since none of the known MSL components are sufficient for direct DNA recognition in vitro, the Larschan laboratory has identified an additional DNA binding protein as a key player in MSL complex recruitment. This previously unstudied zinc-finger protein, CLAMP, (Chromatin Linked Adaptor for MSL Proteins), co-localizes to the X chromosome with MSL complex in vivo. In order to fully investigate the role of CLAMP in dosage compensation, it is necessary to generate a null or mutant of CLAMP. The aim of my project is to generate a CLAMP null mutant using the CRISPR/CAS9 system. With this cutting edge technique, I have site-specifically mutagenized CLAMP in vitro in Drosophila melanogaster S2 and Kc cells and in vivo in Drosophila melanogaster flies.</mods:abstract>
  <mods:name type="personal">
    <mods:namePart>Doherty, Caroline</mods:namePart>
    <mods:role>
      <mods:roleTerm>creator</mods:roleTerm>
    </mods:role>
  </mods:name>
  <mods:name type="corporate">
    <mods:namePart>Brown University. Undergraduate Teaching and Research Awards</mods:namePart>
    <mods:role>
      <mods:roleTerm>research program</mods:roleTerm>
    </mods:role>
  </mods:name>
  <mods:name type="personal">
    <mods:namePart>Larschan, Erica</mods:namePart>
    <mods:role>
      <mods:roleTerm>advisor</mods:roleTerm>
    </mods:role>
    <mods:affiliation>Brown University. Department of Molecular Biology, Cellular Biology and Biochemistry</mods:affiliation>
  </mods:name>
  <mods:originInfo>
    <mods:publisher>Brown University</mods:publisher>
    <mods:dateCreated encoding="w3cdtf">2014-08-07</mods:dateCreated>
    <mods:place>
      <mods:placeTerm type="text">Providence</mods:placeTerm>
    </mods:place>
  </mods:originInfo>
  <mods:genre authority="aat">posters</mods:genre>
  <mods:subject authority="local">
    <mods:topic>Genetics</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>CRISPR</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>CLAMP</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Cas9</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Mutant</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Male specific lethal</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Larschan</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Zinc-finger</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Dosage compensation</mods:topic>
  </mods:subject>
  <mods:subject authority="local">
    <mods:topic>Drosophila</mods:topic>
  </mods:subject>
<mods:identifier type="doi">10.26300/acj1-rb64</mods:identifier><mods:typeOfResource>text</mods:typeOfResource></mods:mods>