- Title Information
- Title
- Microscale Transport Mechanisms: Effect of
Electrokinetic and Interfacial Interactions
- Name:
Personal
- Name Part
- Azadi, Glareh
- Role
- Role Term:
Text
- creator
- Origin Information
- Copyright Date
- 2013
- Physical Description
- Extent
- xxi, 143 p.
- digitalOrigin
- born digital
- Note
- Thesis (Ph.D. -- Brown University (2013)
- Name:
Personal
- Name Part
- Tripathi, Anubhav
- Role
- Role Term:
Text
- Director
- Name:
Personal
- Name Part
- Hurt, Robert
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Hoffman-Kim, Diane
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wessel, Gary
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Maxey, Martin
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Genre (aat)
- theses
- Abstract
- Electrokinetic transport, as a non-mechanical tool in driving the fluid is widely used
in microfluidics. This mode of transport is highly desirable due to simple integration,
precise flow control by an external electric field and applicability over a wide range of
sample conductivities. Fluid transport by electrokinetic techniques is dominated by
surface and interfacial interactions through electrostatic attractions around charged
particles(electrophoresis) or adjacent to the channel surface(electroosmosis). Miniaturization
at microscale provides a high surface to volume ratio where the surface
forces and interfacial effects are significantly enhanced compare to macroscale geometries.
For a successful design of an optimum microfluidic device, these interactions
need to be precisely quantified and controlled. This thesis focuses on studying the
interfacial and electrokinetic interactions in micro-geometries, with the goal of designing
an optimal platform for separation and detection of biomolecules. First, the
interaction of proteins with surfactant molecules was studied to address some of the
fundamental issues in microchip electrophoresis such as simultaneous quantification
and detection. This work was followed by developing a rapid method for detection
and quantification of proteins by electrophoresis and immunodepletion techniques. In
order to quantify the effect of surfactant adsorption on electrokinetic flow, electroosmotic
mobility measurements were performed at the solid-liquid interface of plastic
microcapillaries(poly methylmethacrylate, PMMA). In addition, the electrokinetic
effects with respect to electroporation of the cell wall were applied to develop an
effective technique for inhibition of gram-negative bacteria(E.Coli). Finally, the dispersion
of a semi-infinite suspension of particles was investigated in a capillary, in
order to study the effects of hydrodynamic interactions and capillary confinement on
the diffusive behavior of the particles.
- Subject
- Topic
- Electrokinetic flow
- Subject
- Topic
- Protein-surfactant
- Subject (FAST)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/1079703")
- Topic
- Protein-based surfactants
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20141006
- Language
- Language Term:
Code (ISO639-2B)
- eng
- Language Term:
Text
- English
- Identifier:
DOI
- 10.7301/Z0TM78FH
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Type of Resource (primo)
- dissertations