<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" ID="etd1229" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd">
    <mods:titleInfo>
        <mods:title>Transcriptional regulation of Drosophila melanogaster courtship behavior by fruitless and dissatisfaction</mods:title>
    </mods:titleInfo><mods:name type="personal">
        <mods:namePart>Gyuris, Aron </mods:namePart>
    <mods:role>
        <mods:roleTerm type="text">creator</mods:roleTerm>
    </mods:role>
    </mods:name>
<mods:originInfo>
    <mods:copyrightDate>2014</mods:copyrightDate>
</mods:originInfo>
<mods:physicalDescription>
        <mods:extent>vi, 124 p.</mods:extent>
        <mods:digitalOrigin>born digital</mods:digitalOrigin>
</mods:physicalDescription>
<mods:note>Thesis (Ph.D. -- Brown University (2014)</mods:note>
<mods:name type="personal">
<mods:namePart>McKeown, Michael</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Director</mods:roleTerm>
</mods:role>
</mods:name>

<mods:name type="personal">
<mods:namePart>Reenan, Robert</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>

<mods:name type="personal">
<mods:namePart>Freiman, Richard</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>

<mods:name type="personal">
<mods:namePart>Barnea, Gilad</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>

<mods:name type="personal">
<mods:namePart>Marr, Michael</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="corporate">
        <mods:namePart>Brown University. BIOMED: Molecular Biology, Cell Biology, and Biochemistry</mods:namePart>
        <mods:role>
            <mods:roleTerm type="text">sponsor</mods:roleTerm>
        </mods:role>
        </mods:name>
    <mods:genre authority="aat">theses</mods:genre>
    <mods:abstract>Genetically determined, fixed action pattern behaviors offer a useful model for studying how developmental gene expression programs lay down a neuroanatomical architecture that underlies these behaviors.  Male courtship in Drosophila melanogaster is a robust, tractable behavior governed by a well-defined gene hierarchy.  Within this developmental program, fruitless (fru) and dissatisfaction (dsf) have been identified as regulatory genes whose disruption adversely affects courtship behavior.  fru is complex gene, exhibiting both sexually dimorphic expression and alternative 3’ splicing of putative zinc-finger containing exons.  It is hypothesized that the four different isoforms (A, B, C and D) arising from this splicing direct distinct gene expression programs that give rise to courtship behavior in males, but not females.  dsf is an orphan nuclear hormone receptor that is expressed in one sexually monomorphic form in both sexes.  Despite this similarity, dsf mutation results in loss of courtship in males and gain of courtship in females.  Established as a transcriptional regulator, dsf likely acts on target genes differentially in the sexes through interaction with an undetermined ligand and/or transcriptional cofactors.  To study the roles of fru and dsf in establishing and maintaining the neural circuitry responsible for courtship behavior, we have established epitope-tagged fly lines and cloned versions of the genes for in vivo and in vitro experiments, respectively.  Successful targeting of the fru A, B, C and D and dsf loci individually by homologous recombination yielded animals unaffected phenotypically, yet recapitulating the known expression pattern of both genes.  Although informative in immunohistochemical localization experiments, the endogenous levels of tagged fru or dsf proved inadequately low to yield sufficient interacting DNA for sequencing and subsequent target gene analysis.  Alternatively, dsf was found to bind a known consensus sequence in vitro through an unbiased SELEX approach.  The found dsf binding sequence was validated through mobility shift.  This specific protein-DNA interaction guides future examination of the genes dsf regulates in directing a sex-specific developmental program that establishes the neural circuit responsible for male courtship behavior and female receptivity.  </mods:abstract>

    <mods:subject>
        <mods:topic>Courtship Behavior</mods:topic>
    </mods:subject>

    <mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/898388"><mods:topic>Drosophila</mods:topic></mods:subject><mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/1199312"><mods:topic>Courtship in animals</mods:topic></mods:subject><mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/1154563"><mods:topic>Transcription</mods:topic></mods:subject><mods:recordInfo>
        <mods:recordContentSource authority="marcorg">RPB</mods:recordContentSource>
        <mods:recordCreationDate encoding="iso8601">20141006</mods:recordCreationDate>        
    </mods:recordInfo>
<mods:language xmlns:xlink="http://www.w3.org/1999/xlink"><mods:languageTerm type="code" authority="iso639-2b">eng</mods:languageTerm><mods:languageTerm type="text">English</mods:languageTerm></mods:language><mods:identifier xmlns:xlink="http://www.w3.org/1999/xlink" type="doi">10.7301/Z0QJ7FNN</mods:identifier><mods:accessCondition xmlns:xlink="http://www.w3.org/1999/xlink" type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition><mods:accessCondition type="restriction on access">Collection is open for research.</mods:accessCondition><mods:typeOfResource xmlns:xlink="http://www.w3.org/1999/xlink" authority="primo">dissertations</mods:typeOfResource></mods:mods>