Microfluidic technology provides a means of precisely controlling fluid flow for molecular analytics and diagnostics, as well for the biological analysis of cells and small organisms. By spatially and temporally controlling concentrations and cells, new fundamental tools for biological and clinical discovery can be developed and improved. With the goal of exploring applications of microfluidic technology, first by fundamentally understanding the underlying physics at the micro-scale, and following through to application, this work explores the unique physical and biochemical parameters associated with microfluidic and molecular assay design as well as application to relevant physical, biological, and medical questions. Thus, this thesis focuses on three distinct thrusts in microfluidics; creating technology for improving molecular diagnostics for Clostridium difficile and influenza A using point-of-care methods, evaluating and understanding the physical parameters of droplet based flows in microsystems, and hydrodynamically isolating and analyzing oocytes and embryos for the study of developmental and reproductive biology.
Angione, Stephanie L.,
"Microfluidic Platforms for Molecular Diagnostics and Developmental Biology"
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.