Title Information
Title
The Effect of Confinement on Neutrophil Force Generation
Name: Personal
Name Part
Toyjanova, Jennet
Role
Role Term: Text
creator
Origin Information
Copyright Date
2015
Physical Description
Extent
xxii, 123 p.
digitalOrigin
born digital
Note
Thesis (Ph.D. -- Brown University (2015)
Name: Personal
Name Part
Franck, Christian
Role
Role Term: Text
Director
Name: Personal
Name Part
Bower, Allan
Role
Role Term: Text
Reader
Name: Personal
Name Part
Hammer, Daniel
Role
Role Term: Text
Reader
Name: Personal
Name Part
Kim, Kyung-Suk
Role
Role Term: Text
Reader
Name: Personal
Name Part
Reichner, Jonathan
Role
Role Term: Text
Reader
Name: Corporate
Name Part
Brown University. ENGINEERING: Mechanics of Solids
Role
Role Term: Text
sponsor
Genre (aat)
theses
Abstract
Generation of forces is essential for the migration of neutrophils to the sites of infection or injury. The mechanical properties and local confinement of the surrounding microenvironment play a significant role in regulating these forces. However, it is challenging to decouple each of these features experimentally without inherently changing the microstructure of the cell environment. Presented here is an experimental method that is used to isolate confinement and substrate stiffness as single variables in the neutrophil environment. Using a combination of fast iterative digital volume correlation and traction force microscopy techniques, this dissertation demonstrates that neutrophils induce finite displacements on their surroundings. Furthermore, the findings show that neutrophil-generated forces are highly affected by confinement and stiffness of their microenvironment.
Subject
Topic
cell mechanics
Subject (FAST) (authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/850266")
Topic
Cells--Mechanical properties
Record Information
Record Content Source (marcorg)
RPB
Record Creation Date (encoding="iso8601")
20150601
Language
Language Term: Code (ISO639-2B)
eng
Language Term: Text
English
Identifier: DOI
10.7301/Z0TM78GZ
Access Condition: rights statement (href="http://rightsstatements.org/vocab/InC/1.0/")
In Copyright
Access Condition: restriction on access
Collection is open for research.
Type of Resource (primo)
dissertations