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<mods:titleInfo>
<mods:title>Three MYB transcription factors coordinate expression of an array of secreted proteins that promote pollen tube identity and reproductive cell signaling.</mods:title>
</mods:titleInfo><mods:name type="personal">
<mods:namePart>Leydon, Alexander Rivinus</mods:namePart>
<mods:role>
<mods:roleTerm type="text">creator</mods:roleTerm>
</mods:role>
</mods:name>
<mods:originInfo>
<mods:copyrightDate>2015</mods:copyrightDate>
</mods:originInfo>
<mods:physicalDescription>
<mods:extent>24, 452 p.</mods:extent>
<mods:digitalOrigin>born digital</mods:digitalOrigin>
</mods:physicalDescription>
<mods:note>Thesis (Ph.D. -- Brown University (2015)</mods:note>
<mods:name type="personal">
<mods:namePart>Johnson, Mark</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Director</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Bender, Judith</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>DeLong, Alison</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Larschan, Erica</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="personal">
<mods:namePart>Kessler, Sharon</mods:namePart>
<mods:role>
<mods:roleTerm type="text">Reader</mods:roleTerm>
</mods:role>
</mods:name>
<mods:name type="corporate">
<mods:namePart>Brown University. BIOMED: Molecular Biology, Cell Biology, and Biochemistry</mods:namePart>
<mods:role>
<mods:roleTerm type="text">sponsor</mods:roleTerm>
</mods:role>
</mods:name>
<mods:genre authority="aat">theses</mods:genre>
<mods:abstract>Successful plant reproduction is the lynchpin of agricultural food production. With high susceptibility to climatic variations and other stress conditions, plant reproduction demands further investigation into its mechanisms, especially pollen and pistil signaling pathways that can be optimized for seed production. During pollination a series of pollen tube-pistil interactions ensure that the right sperm get to the right egg to deliver sperm for fertilization. In Arabidopsis, the reception of a pollen tube into an ovule initiates with a cell:cell interaction between the pollen tube and the synergid cell, which culminates in sperm release.
We define a trio of R2R3-MYB transcription factors (MYB97, MYB101, MYB120) that are expressed in pollen tubes and are essential for pollen tube reception. myb triple-mutant pollen tubes fail to stop growing and burst within the female gametophyte, accordingly the genes regulated by these transcription factors are candidates for pollen tube components of the pollen tube reception mechanism. We developed a novel SNP-informed RNA-sequencing method to simultaneously identify pollen and pistil gene expression profiles from inter-ecotype crosses between Cape Verde Island (Cvi) and Columbia (Col) Arabidopsis accessions. We applied this method to comprehensively define genes regulated by MYBs in the pollen tube, which were enriched in three categories: carbohydrate active proteins, transmembrane transporters, and small secreted proteins. We demonstrate that two MYB-regulated small secreted proteins promote pollen tube reception; a Thionin (CRP2460), and an S protein homolog (SPH). Thionins are secreted from the pollen tube to promote synergid degeneration during reception. One SPH protein is sufficient to increase myb triple-mutant fertility, which is the first pollen gene described that controls pollen tube reception.
Our studies have, for the first time, identified pollen tube genes that create pollen tube identity via extracellular molecular patterns recognized by synergid cells. MYB-regulated pollen tube genes uncover reception signaling pathways that are surprisingly akin to plant defense responses. Both the pollen tube and synergid cells convey bits of information to each other upon cell-cell contact, which constitute what we term a reproduction-associated molecular pattern or RAMP.
</mods:abstract>
<mods:subject>
<mods:topic>cell signaling</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>MYB transcription factors</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>fertilization</mods:topic>
</mods:subject>
<mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/1094973"><mods:topic>Reproduction</mods:topic></mods:subject><mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/1070023"><mods:topic>Pollen tube</mods:topic></mods:subject><mods:subject xmlns:xlink="http://www.w3.org/1999/xlink" authority="FAST" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/923193"><mods:topic>Fertilization (Biology)</mods:topic></mods:subject><mods:recordInfo>
<mods:recordContentSource authority="marcorg">RPB</mods:recordContentSource>
<mods:recordCreationDate encoding="iso8601">20150601</mods:recordCreationDate>
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<mods:language xmlns:xlink="http://www.w3.org/1999/xlink"><mods:languageTerm type="code" authority="iso639-2b">eng</mods:languageTerm><mods:languageTerm type="text">English</mods:languageTerm></mods:language><mods:identifier xmlns:xlink="http://www.w3.org/1999/xlink" type="doi">10.7301/Z01G0JPS</mods:identifier><mods:accessCondition xmlns:xlink="http://www.w3.org/1999/xlink" type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition><mods:accessCondition type="restriction on access">Collection is open for research.</mods:accessCondition><mods:typeOfResource xmlns:xlink="http://www.w3.org/1999/xlink" authority="primo">dissertations</mods:typeOfResource></mods:mods>