Analysis of an ACC hypersensitive A. thaliana mutant

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Title
Analysis of an ACC hypersensitive A. thaliana mutant
Contributors
Corcoran, Emma (creator)
DeLong, Alison (advisor)
Brown University. Undergraduate Teaching and Research Award (research program)
Doi
10.26300/tgeq-kh68
Date Created
2015-08-07
Abstract
Ethylene is a gaseous hormone that plays an important role in many cellular processes in plants, including the promotion of fruit ripening and the inhibition of cell elongation. We have identified an Arabidopsis thaliana mutant that is hypersensitive to ACC, an ethylene precursor. This ACC Hypersensitive Hypocotyl 1 (ahh1) mutant has short hypocotyls on untreated MS1 media and is rescued by AVG treatment. In addition, it shows an exaggerated response to exogenous ACC compared to the wild-type, Col-0. Our goal is to characterize this mutant's lesion in the ethylene reception pathway by determining whether ahh1 follows the "eer" ethylene induction pattern or the canonical hypersensitive pattern. Using quantitative RT-PCR, we found that ahh1 shows neither "eer" nor canonical induction behavior. From this information, we conclude that we are characterizing an unmapped ethylene lesion. Since AgNO3 treatment did not completely inhibit ACC induction in ahh1, the ahh1 mutation likely affects a component after ETR1 in the ethylene reception pathway. Moreover, ahh1 seedlings show a weak ctr-like phenotype. These data suggest that CTR1 may be affected in the ahh1 mutant.
Keywords
Ethylene
Arabidopsis thaliana
Extent
1 poster

Citation

Corcoran, Emma, "Analysis of an ACC hypersensitive A. thaliana mutant" (2015). Summer Research Symposium. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/tgeq-kh68

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