Cellular immunotherapy is a type of treatment available to cancer patients where the immune system is utilized to combat tumors. In a previous clinical trial, patients who were given this treatment were infused with haploidentical CD3+ cells at 1-2x10^8 CD3+ cells/kg. Over half of these patients developed major responses with all of them developing a fever of 104°F. In order to determine what specific variables made some responses more effective than others, we looked at different factors that could be affecting them-one of them being fever. In previous studies, fever has been found to incur beneficial effects to patients and so we focused on testing whether fever could have a positive influence on alloreactive immune responses. In order to investigate this, we used mixed lymphocyte cultures (MLCs) incubated in duplicate at 37°C and 40°C for varying incubation periods with a few different stimulators. We measured cell proliferation and cytotoxicity to determine whether fever proved to enhance the immune response. Preliminary results show that exposure of cells to 40°C seems to inhibit the immune response by perhaps killing off T lymphocytes.
"The impact of febrile temperature on alloreactive immune responses"
Summer Research Symposium.
Brown Digital Repository. Brown University Library.
Each year, Brown University showcases the research of its undergraduates at the Summer Research Symposium. More than half of the student-researchers are UTRA recipients, while others receive funding from a variety of Brown-administered and national programs and fellowships and go …