Title Information
Title
Characterizing DNA secondary structure of hairpin-containing intermediates of the base excision repair pathway
Name: Personal
Name Part
Bolton, Elizabeth
Role
Role Term: Text
creator
Name: Personal
Name Part
Delaney, Sarah
Role
Role Term: Text
advisor
affiliation
Brown University. Department of Chemistry
Name: Corporate
Name Part
Brown University. Undergraduate Teaching and Research Award
Role
Role Term: Text
research program
Type of Resource
still image
Genre (aat)
posters
Origin Information
Place
Place Term: Text
Providence
Publisher
Brown University
Date Created (encoding="w3cdtf")
2015-08-07
Physical Description
Extent
1 poster
digitalOrigin
reformatted digital
Abstract
Regions of repetitive DNA, in particular trinucleotide repeats, are common throughout the human genome and are of interest as a source of genomic instability. DNA strands that have trinucleotide repeats, such as (CAG)n repeats can expand to yield a longer repeat tract. While the exact mechanism of expansion is still unknown, expansion is hypothesized to result from the ability of (CAG)n repeat DNA to form non-canonical secondary structures, including stem-loop hairpins. Hairpins are "hotspots" for DNA damage because the unpaired guanine in the loop of the hairpin is subject to oxidation, leading to an 8-oxo- 7,8 dihydroguanine lesion, which must be repaired. This project is focused on the Base Excision Repair (BER) pathway, which is responsible for repairing these oxidized base lesions. Within mixed sequence DNA, the enzymes involved in BER remove damaged bases and replace them to restore the integrity of the DNA sequence. DNA Ligase 1 typically completes the repair event, creating a new phosphodiester bond at the nick site in the DNA. However, (CAG)n repeat DNA leads to hairpin-containing intermediates which contain extra nucleotides. If DNA Ligase 1 is able to ligate these hairpin-containing strands, the repeat tract will be expanded. My project will be using chemical probes that react with solvent exposed bases to sequence and characterize the secondary structure of proposed hairpin-containing intermediates of the Base Excision Repair pathway.
Subject (LCSH)
Topic
DNA
Subject (LCSH)
Topic
Biochemistry
Identifier: DOI
10.26300/e1gz-3s08