Title Information
Title
Achieving bacteriostasis using diamide inhibitors of bacterial GlcNAcases
Name: Personal
Name Part
Jamieson, Mitchell
Role
Role Term: Text
creator
Name: Personal
Name Part
Basu, Amit
Role
Role Term: Text
advisor
affiliation
Brown University. Department of Chemistry
Name: Corporate
Name Part
Brown University. LINK Award
Role
Role Term: Text
research program
Type of Resource
still image
Genre (aat)
posters
Origin Information
Place
Place Term: Text
Providence
Publisher
Brown University
Date Created (encoding="w3cdtf")
2015-08-07
Physical Description
Extent
1 poster
digitalOrigin
reformatted digital
Abstract
Last year, the World Health Organization classified antimicrobial resistance as a "serious, worldwide threat to public health." Newly developed antibiotics must therefore circumvent the known mechanisms of bacterial resistance for better efficacy. The Basu Laboratory has discovered a new class of small molecules that inhibit bacterial cell wall recycling and remodeling enzymes that act on the invariable glycan backbone of the peptidoglycan heteropolymer. Several of these compounds have demonstrated bacteriostatic activity against Bacillus subtilis. The most potent inhibitors are dipeptides that were synthesized using the Ugi multicomponent condensation reaction. Resazurin whole cell assays have proven successful, and preliminary isothermal titration calorimetry experiments utilizing the enzyme target LytG (exo-acting GlcNAcase) are promising. With a large compound library in its third generation, we aim to improve the potency of these inhibitors through determination of which moieties hinder bacterial growth most.
Subject (LCSH)
Topic
Drug resistance in microorganisms
Subject (LCSH)
Topic
Peptidoglycans
Subject (LCSH)
Topic
Resazurin
Identifier: DOI
10.26300/kzze-wy65