- Title Information
- Title
- CRISPR genome editing in sea urchins
- Name:
Personal
- Name Part
- Shevidi, Saba
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Yajima, Mamiko
- Role
- Role Term:
Text
- advisor
- affiliation
- Brown University. Department of Molecular Biology, Cell Biology, and
Biochemistry
- Name:
Personal
- Name Part
- Wessel, Gary
- Role
- Role Term:
Text
- advisor
- affiliation
- Brown University. Department of Molecular Biology, Cell Biology, and
Biochemistry
- Name:
Corporate
- Name Part
- Brown University. Undergraduate Teaching and Research Award
- Role
- Role Term:
Text
- research program
- Type of Resource
- still image
- Genre (aat)
- posters
- Origin Information
- Place
- Place Term:
Text
- Providence
- Publisher
- Brown University
- Date Created
(encoding="w3cdtf")
- 2015-08-07
- Physical Description
- Extent
- 1 poster
- digitalOrigin
- reformatted digital
- Abstract
- CRISPR is a bacterial adaptive immune responsive against invading viruses
consisting of endonuclease Cas9 and two guide RNAs known as sgRNAs. Its application can
cause mutations and, with proper manipulation, edit the genome of organisms, which has been
demonstrated in a variety of organisms. However, CRISPR technology has not been tested in
sea urchins. We investigated CRISPR genome editing efficiency in S. purpuratus, and further
used its application to study the novel enzyme deaminase. CRISPR showed to have a net
efficiency of GFP reporter knockdown. Thus, CRISPR is effective in sea urchins although its
full potential has not been achieved. We next explored the genome editing efficiency of
novel enzyme deaminase by fusing it with Cas9. If effective, this would cause the urchin
genome to be edited at a single nucleotide level from C->T. Early testing of this enzyme has
shown to be slightly effective, although experiments to test its efficiency are still being
conducted.
- Subject (LCSH)
- Topic
- Sea urchins
- Subject (LCSH)
- Topic
- Immune system
- Subject (LCSH)
- Topic
- Endonucleases
- Identifier:
DOI
- 10.26300/c60z-2e44