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Inter-alpha inhibitor proteins reduce neutrophil infiltration in the brain after hypoxic-ischemic injury in the neonate


Introduction: We have previously shown that inter-alpha inhibitor proteins (IAIPs), immunomodulatory proteins that play a significant anti-inflammatory role in hypoxic ischemic (HI) injury, improve histopathological brain injury, brain weight, and behavioral outcomes in neonatal rats. Neutrophils are leukocytes that infiltrate the brain parenchyma and exacerbate neuronal injury after HI. Objective: To determine the effect of IAIPs on neutrophil infiltration in neonatal rats after HI. Methods: The Vannucci model was used to induce neonatal HI in postnatal day 7 rats that were assigned to a Non-ischemic sham-control group (Sham, n=12), a right-side carotid ligation with exposure to hypoxia (8% oxygen for 90 min) treated with placebo group (PL-HI, n=17), or an IAIP treated group (IAIP-HI, n=17). Rat sex was recorded. IAIP (30 mg/kg) or PL was given intraperitoneally at 0, 24 and 48 h after HI. 72 h after HI, brains were removed and prepared for analysis. We performed immunohistochemistry by treating brain tissue with MPO (a neutrophil selective marker). We performed stereological analyses with the StereoInvestigator 10.0, Fractionator probe without knowledge of group assignment to quantify neutrophils present within the right hemisphere, cortex, corpus callosum, and hippocampus per area. Results: MPO positive cells were significantly reduced in male IAIP treated rats compared with PL-HI in the the overall damaged hemisphere (p<0.01) and in the corpus callosum (p<0.05).\n\n


Barrios-Anderson, Adriel, and Chen, Xiaodi, "Inter-alpha inhibitor proteins reduce neutrophil infiltration in the brain after hypoxic-ischemic injury in the neonate" (2016). Summer Research Symposium. Brown Digital Repository. Brown University Library.



  • Summer Research Symposium

    Each year, Brown University showcases the research of its undergraduates at the Summer Research Symposium. More than half of the student-researchers are UTRA recipients, while others receive funding from a variety of Brown-administered and national programs and fellowships and go …