<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" ID="bdr697272" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/mods/v3/mods-3-6.xsd">
   <mods:titleInfo>
      <mods:title>Biophysical characterization of the RRP1B-PP1 holoenzyme</mods:title>
   </mods:titleInfo>
   <mods:name type="personal">
      <mods:namePart>Cho, Haejun</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">creator</mods:roleTerm>
      </mods:role>
   </mods:name>
   <mods:name type="personal">
      <mods:namePart>Bajaj, Rakhi</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">creator</mods:roleTerm>
      </mods:role>
   </mods:name>
   <mods:name type="personal">
      <mods:namePart>Ganesan, Senthill</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">creator</mods:roleTerm>
      </mods:role>
      <mods:affiliation>Brown University. Department of Molecular Pharmacology, Physiology and Biotechnology</mods:affiliation>      
   </mods:name>
   <mods:name type="personal">
      <mods:namePart>Peti, Wolfgang</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">advisor</mods:roleTerm>
      </mods:role>
      <mods:affiliation>Brown University. Department of Molecular Pharmacology, Physiology, and Biotechology</mods:affiliation>
   </mods:name>
   <mods:name type="personal">
      <mods:namePart>Ganesan, Senthil</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">advisor</mods:roleTerm>
      </mods:role>
      <mods:affiliation>Brown University. Department of Molecular Pharmacology, Physiology, and Biotechology</mods:affiliation>
   </mods:name>
   <mods:name type="personal">
      <mods:namePart>Page, Rebecca</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">creator</mods:roleTerm>
      </mods:role>
      <mods:affiliation>Brown University. Department of Molecular Biology, Cell Biology, and Biochemistry</mods:affiliation>
   </mods:name>
   <mods:name type="corporate">
      <mods:namePart>Brown University. Undergraduate Teaching and Research Award</mods:namePart>
      <mods:role>
         <mods:roleTerm type="text">research program</mods:roleTerm>
      </mods:role>

   </mods:name>
   <mods:genre authority="aat">posters</mods:genre>
   <mods:originInfo>
      <mods:place>
         <mods:placeTerm type="text">Providence, RI</mods:placeTerm>
      </mods:place>
      <mods:publisher>Brown University</mods:publisher>
      <mods:dateCreated keyDate="yes" encoding="w3cdtf">2016</mods:dateCreated>
   </mods:originInfo>
   <mods:physicalDescription>
      <mods:extent>1 poster</mods:extent>
      <mods:digitalOrigin>reformatted digital</mods:digitalOrigin>
   </mods:physicalDescription>
   <mods:abstract>Protein Phosphatase 1 (PP1) is one of the most ubiquitous phosphatases that are found in all eukaryotic cells. PP1 binds with more than 200 known regulatory proteins to determine its substrate specificity. Almost all PP1 interactors have the canonical RVxF motif, which is required to bind to the RVxF hydrophobic pocket on PP1, as well as additional PP1-interacting motifs to enhance binding. Ribosomal RNA processing 1 homolog B (RRP1B) targets PP1 to the nucleolus and is involved in the processing of the pre-60S ribosomal subunit. Since there are not many known nucleolar targeting proteins for PP1, characterizing the interaction between RRP1B and PP1 will help us to understand different targeting mechanisms and ultimately to unravel the PP1 code. Here we demonstrate that RRP1B shows strong differential binding for two isoforms of PP1, PP1 gamma and PP1 alpha, using Isothermal Titration Calorimetry. We also show that the RRP1B construct we used for this experiment is intrinsically disordered using 1H-15N-Heteronuclear Single Quantum Coherence spectroscopy. </mods:abstract>
   <mods:subject authority="lcsh">
      <mods:topic>Phosphoprotein phosphatases</mods:topic>
   </mods:subject>
   <mods:subject authority="lcsh">
      <mods:topic>Nuclear magnetic resonance</mods:topic>
   </mods:subject>

<mods:identifier xmlns:xlink="http://www.w3.org/1999/xlink" type="doi">10.26300/872q-0z92</mods:identifier><mods:typeOfResource xmlns:xlink="http://www.w3.org/1999/xlink">text</mods:typeOfResource></mods:mods>