- Title Information
- Title
- Group 1 Innate Lymphoid Cells of the Submandibular Salivary Gland and Lacrimal Gland
- Name:
Personal
- Name Part
- Erick, Timothy
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Brossay, Laurent
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Atwood, Walter
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wands, Jack
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Sharma, Surendra
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Lynch, Lydia
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Department of Molecular Biology, Cell Biology and Biochemistry
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2017
- Physical Description
- Extent
- x, 186 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Ph. D.)--Brown University, 2017
- Genre (aat)
- theses
- Abstract
- Innate lymphoid cells (ILCs) are diverse innate lymphocytes that contribute to the immune response against pathogens and cancer cells, and play a variety of roles in tissue homeostasis. ILCs are divided into three groups: ILC1s, ILC2s, and ILC3s. ILC1s include conventional NK (cNK) cells, which kill virally infected cells and tumor cells and produce pro-inflammatory cytokines. In addition, unique populations of tissue-resident NK (trNK) cells have been identified in several different tissues in mice and humans. ILC1 populations in the submandibular salivary gland (SMG) and lacrimal gland (LG) have not been extensively characterized. The SMG and LG are exocrine tissues that are crucial for oral and corneal health, respectively. The anatomical locations of these glands means they are likely to come into contact with a variety of microorganisms. NK cells are crucial for early defense against pathogens. We used the mouse as a model system to study the phenotype and functions of NK cells in the SMG and LG. NFIL3 is a transcription factor that is required for the development of cNK cells, but not for most trNK cell populations. Using NFIL3-deficient mice, mixed bone marrow chimeras, and fate mapping mice, we demonstrated that the SMG contains two populations of NK cells: a majority population of cNK-like cells, and a minority population of NFIL3-independent trNK cells. Our lab has previously shown that SMG NK cells are hyporesponsive to mouse CMV (MCMV) infection. Using an adoptive transfer approach, we demonstrated that the hyporesponsive phenotype of SMG cNK-like cells is a tissue-specific property. In contrast, SMG NFIL3-independent trNK cells are intrinsically hyporesponsive. We also observed a novel population of innate-like T cells in the SMG of NFIL3-deficient mice. Further work will be necessary to characterize their development and function. We also showed that the LG contains a population of NK cells. LG NK cells appear to be conventional in development, and they respond weakly to systemic MCMV infection. Much like SMG cNK-like cells, the hyporesponsive phenotype of LG NK cells is tissue-specific. Altogether, the results of this thesis demonstrate that exocrine glands contain unique populations of NK cells.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00968006")
- Topic
- Immunology
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00850281")
- Topic
- Cellular immunity
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01034361")
- Topic
- Natural immunity
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00967867")
- Topic
- Immune response
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00967877")
- Topic
- Immune system
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20170616
- Identifier:
DOI
- 10.7301/Z03T9FNK
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Type of Resource (primo)
- dissertations