Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis

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Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis
Thelwell, Jeray Anthony (creator)
Tripathi, Anubhav (Advisor)
Breuer, Kenneth (Reader)
Mathiowitz, Edith (Reader)
Wessel, Gary (Reader)
Brown University. Biology and Medicine: Biomedical Engineering (sponsor)
Copyright Date
The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly, diagnostic technology must have predictive potential. As medical education continues to develop with the growing body of basic biological research, there is increased emphasis on preventative medicine. Instead of training more highly skilled surgical specialty doctors to mechanically remedy exacerbated ailments of the body, lives are now being saved before patients even make it into the operating room. Predictive diagnostic technologies provide a wealth of information, not only about current pathological status, but also about the real-time effects of treatment with little to no harm of the patient. Secondly, diagnostic technology must offer personalized patient information. With completion of the human genome project and the advent of more sophisticated sequencing modalities, medical care tailored to specific individuals is more of a possibility. A greater understanding of epigenetic modulation of the genome is promoting the transition from “one size fits all” care. While approaching this problem in the Tripathi Lab, two additional constraints were imposed: the diagnostic technology must be robust yet economical and simple to use in order to allow for widespread adoption. To that end, a microfluidic platform for the extraction of short fragment DNA from crowded solution is reported with the idea of mimicking cell-free DNA found in blood. Preliminary off-chip experiments comparing different magnetic beads to determine the optimal bead and buffer composition for short DNA fragments are first completed. Then an investigation into multi-bead systems for size based extraction through the oil-water interface is carried out. Finally, on-chip short fragment DNA recovery is demonstrated from buffer solution and spiked human serum solution.
Microfluidic devices
DNA extraction
Thesis (Sc. M.)--Brown University, 2017
ix, 43 p.


Thelwell, Jeray Anthony, "Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis" (2017). Biomedical Engineering Theses and Dissertations. Brown Digital Repository. Brown University Library.