This is a movie showing bioluminescence in left primary somatosensory cortex in a mouse expressing the LMO3 molecule. Water-Soluble Coelenterazine (CTZ) by Nanolight was injected directly in the cortex. The concentration was 1μg/μl (2.34 mM concentration, volume 2.5 μl). The animal was anesthetized.
Notes:
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging is funded by National Science Foundation Award #1707352. This research received funding from the NIH National Institute of Neurological Disorders and Stroke (NINDS) Award U01NS099709 and funding from the W. M. Keck Foundation (Bioluminescent Optogenetics to Autoregulate Excitable Cells).
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Citation
Gomez-Ramirez, Manuel,
"Movie: Bioluminescence when injecting coelenterazine directly in cortex (1/2)"
(2017).
NeuroNex Data and Research Products.
Brown Digital Repository. Brown University Library.
https://doi.org/10.7301/Z0GB22JM
This collection contains publicly available research products, including data, codes, and publications, among others. The NeuroNex Technology Hub is supported by the National Science Foundation (NSF) and its Division of Biological Infrastructure under Award No. 1707352.