Title Information
Title
Movie: Calcium dependent bioluminescence when applying coelenterazine systemically through lateral ventricle and a calcium agonist directly in cortex (2/2)
Abstract
This is a movie showing Calcium Dependent Bioluminescence in a mouse expressing LMC4 in left primary somatosensory cortex. Water soluble Coelenterazine (CTZ) was injected in the lateral ventricle of the right hemisphere, while the calcium agonist (NMDA) was injected directly in cortex. The CTZ concentration was 1μg/μl (2.34 mM concentration, volume 2.5 μl). Bioluminescence was maximal when both CTZ and NMDA were present at the same time. The animal was anesthetized.
Name
Name Part
Gomez-Ramirez, Manuel
Role
Role Term (marcrelator) (authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
Creator
Origin Information
Date Created
2017-08-15
Type of Resource
moving image
Genre
movie
Note: funding
NeuroNex Technology Hub: Bioluminescence for Optimal Brain Control and Imaging is funded by National Science Foundation Award #1707352. This research received funding from the NIH National Institute of Neurological Disorders and Stroke (NINDS) Award U01NS099709 and funding from the W. M. Keck Foundation (Bioluminescent Optogenetics to Autoregulate Excitable Cells).
Access Condition: use and reproduction (href="https://creativecommons.org/licenses/by-nc-nd/4.0/")
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Access Condition: logo (href="https://licensebuttons.net/l/by-nc-nd/4.0/88x31.png")
Identifier: DOI
10.7301/Z0348HW8
Subject (Local)
Topic
bioluminescent optogenetics
Subject (Local)
Topic
BL-OG
Subject (Local)
Topic
Bioluminescence
Subject (Local)
Topic
coelenterazine
Subject (Local)
Topic
somatosensory cortex
Subject (Local)
Topic
LMC4
Subject (Local)
Topic
lumicampsin