Thyroid Microtissues Are a Precursor to a Tissue Engineered Platform Tor Toxicity Testing

Hopkins, Caitlin Marie

Full Metadata

Overview

Year:: 2018
Contributor:: Hopkins, Caitlin Marie (creator); Morgan, Jeffrey (Advisor); Boekelheide, Kim (Reader); Ip, Blanche (Reader); Brown University. Department of Molecular Pharmacology, Physiology and Biotechnology (sponsor)
Genre:: theses
Subject:: tissue engineering; Toxicology
Extent:: 8, 79 p.
DOI: https://doi.org/10.26300/2msk-nf35

Files

Description

Abstract:: Standard toxicity testing of chemicals to assess risk in humans is commonly done on animals. The problem, however, is that animals have been found to have many physiological differences from humans and are not the most predictive models when it comes to assessing human risk. As such, there has been a push to create more predictive in-vitro models. Tissue engineering has allowed for the creation of complex systems that are more replicative of in-vivo environments. Commonly used strategies include implementation of a three-dimensional environment, use of human cell types, and incorporation of appropriate physical and chemical forces; an ideal new toxicity test would utilize all of these factors. Our approach in creating a benchtop thyroid model uses 3D Petri Dishes to create spheroids from an immortalized thyroid human cell line. The function of the thyroid is to produce thyroid hormones, and thus that became the desired function of our platform. To begin characterizing our model, we studied the formation of spheroids over time under varying conditions. Our system incorporates the benefits of a three-dimensional environment, appropriate growth and stimulatory factors, and co-culture with stromal cell types. Techniques including microscopy, histology, and enzyme immunoassay were utilized to study our microtissues. By successfully developing a model using human microtissues capable of producing thyroid hormones, a new in-vitro platform for toxicity testing was created.
Notes:: Thesis (Sc. M.)--Brown University, 2018

Content

Access Conditions

Rights: In Copyright
Restrictions on Use: Collection is open for research.

Citation

Hopkins, Caitlin Marie, "Thyroid Microtissues Are a Precursor to a Tissue Engineered Platform Tor Toxicity Testing" (2018). Molecular Pharmacology, Physiology, and Biotechnology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/2msk-nf35

Relations

Collection:

Molecular Pharmacology, Physiology, and Biotechnology Theses and Dissertations

Theses and Dissertations for the Molecular Pharmacology, Physiology, and Biotechnology department.
...