- Title Information
- Title
- A Biophysical Analysis of Neutrophil Force Generation in a Biochemical Environment
- Type of Resource
- text
- Name:
Personal
- Name Part
- Andrews, Christina
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Franck, Christian
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Lefort, Craig
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Reichner, Jonathan
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wong, Ian
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2018
- Physical Description
- Extent
- i, 1 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2018
- Genre (aat)
- theses
- Abstract
- Neutrophils are the most abundant circulating white blood cell in the human body, and play a crucial role in the innate immune response to infection and inflammation. Sepsis is a systemic bacterial infection that results in a complex immune response. In this event, neutrophils are exposed to an excess of chemical stimuli that results in over activation. These over-activated neutrophils cause tissue damage, organ dysfunction, and death. In this project, I will study the change in cellular mechanisms between naïve and lipopolysaccharide (LPS)-activated neutrophils by quantifying the material displacement fields and surface tractions. This will provide information moving forward to understand the mechanical dysregulation that neutrophils undergo at a heightened activation state. This data will aid our understanding of neutrophil biochemical and mechanical sensing to recognize injury, and then migrate to the site of injury.
In this study, I will identify the change in neutrophil motility and force generation before and after LPS activation. I use human fibronectin and human ICAM-1 coated on mechanically tunable polyacrylamide hydrogels (E=1.7 kPa and 8.7 kPa) to study naïve and LPS-activated neutrophils. By studying material displacement fields and surface tractions, the Franck Lab will better understand healthy and over-activated neutrophil motility and identify key phenotypic markers to detect and provide treatment in the event of sepsis. This will further help establish a baseline on the relationship between mechanics and cellular mechanisms, with a focus on neutrophil migration and adhesion.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00968006")
- Topic
- Immunology
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00967877")
- Topic
- Immune system
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01112835")
- Topic
- Septicemia
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00850266")
- Topic
- Cells--Mechanical properties
- Subject
- Topic
- soft materials
- Subject
- Topic
- traction force microscopy
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20180618
- Identifier:
DOI
- 10.26300/131y-zg77
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.