<mods:mods xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-4.xsd"><mods:titleInfo><mods:title>A Biophysical Analysis of Neutrophil Force Generation in a Biochemical Environment</mods:title></mods:titleInfo><mods:typeOfResource>text</mods:typeOfResource><mods:name type="personal"><mods:namePart>Andrews, Christina</mods:namePart><mods:role><mods:roleTerm type="text">creator</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart>Franck, Christian</mods:namePart><mods:role><mods:roleTerm type="text">Advisor</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart>Lefort, Craig</mods:namePart><mods:role><mods:roleTerm type="text">Reader</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart>Reichner, Jonathan</mods:namePart><mods:role><mods:roleTerm type="text">Reader</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart>Wong, Ian</mods:namePart><mods:role><mods:roleTerm type="text">Reader</mods:roleTerm></mods:role></mods:name><mods:name type="corporate"><mods:namePart>Brown University. Biology and Medicine: Biomedical Engineering</mods:namePart><mods:role><mods:roleTerm type="text">sponsor</mods:roleTerm></mods:role></mods:name><mods:originInfo><mods:copyrightDate>2018</mods:copyrightDate></mods:originInfo><mods:physicalDescription><mods:extent>i, 1 p.</mods:extent><mods:digitalOrigin>born digital</mods:digitalOrigin></mods:physicalDescription><mods:note type="thesis">Thesis (Sc. M.)--Brown University, 2018</mods:note><mods:genre authority="aat">theses</mods:genre><mods:abstract>Neutrophils are the most abundant circulating white blood cell in the human body, and play a crucial role in the innate immune response to infection and inflammation. Sepsis is a systemic bacterial infection that results in a complex immune response. In this event, neutrophils are exposed to an excess of chemical stimuli that results in over activation. These over-activated neutrophils cause tissue damage, organ dysfunction, and death. In this project, I will study the change in cellular mechanisms between naïve and lipopolysaccharide (LPS)-activated neutrophils by quantifying the material displacement fields and surface tractions. This will provide information moving forward to understand the mechanical dysregulation that neutrophils undergo at a heightened activation state. This data will aid our understanding of neutrophil biochemical and mechanical sensing to recognize injury, and then migrate to the site of injury.&#13;
	In this study, I will identify the change in neutrophil motility and force generation before and after LPS activation. I use human fibronectin and human ICAM-1 coated on mechanically tunable polyacrylamide hydrogels (E=1.7 kPa and 8.7 kPa) to study naïve and LPS-activated neutrophils. By studying material displacement fields and surface tractions, the Franck Lab will better understand healthy and over-activated neutrophil motility and identify key phenotypic markers to detect and provide treatment in the event of sepsis. This will further help establish a baseline on the relationship between mechanics and cellular mechanisms, with a focus on neutrophil migration and adhesion.</mods:abstract><mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/00968006"><mods:topic>Immunology</mods:topic></mods:subject><mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/00967877"><mods:topic>Immune system</mods:topic></mods:subject><mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/01112835"><mods:topic>Septicemia</mods:topic></mods:subject><mods:subject authority="fast" authorityURI="http://id.worldcat.org/fast" valueURI="http://id.worldcat.org/fast/00850266"><mods:topic>Cells--Mechanical properties</mods:topic></mods:subject><mods:subject><mods:topic>soft materials</mods:topic></mods:subject><mods:subject><mods:topic>traction force microscopy</mods:topic></mods:subject><mods:language><mods:languageTerm authority="iso639-2b">English</mods:languageTerm></mods:language><mods:recordInfo><mods:recordContentSource authority="marcorg">RPB</mods:recordContentSource><mods:recordCreationDate encoding="iso8601">20180618</mods:recordCreationDate></mods:recordInfo><mods:identifier type="doi">10.26300/131y-zg77</mods:identifier><mods:accessCondition type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition><mods:accessCondition type="restriction on access">Collection is open for research.</mods:accessCondition></mods:mods>