Description
- Abstract:
- Ischemic heart disease is the leading cause of death globally, which takes more than 17.7 million lives every year (WHO, 2018), yet current treatments and procedures cannot restore cardiac tissue lost due to ischemia. One of the goals for regenerative medicine is to use human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) as a renewable cell source to replace lost cardiomyocytes. The most significant limitation to the use of hiPSC-CMs in regenerative medicine is our lack of understanding of their biology, including their maturity state. To address this, a series of experiments were carried out using photolithography and microcontact printing techniques to create single cell patterns for hiPSC-derived cardiomyocytes to examine their structure and development. The specific patterns used in the experiments were rectangles with an aspect ratio of 1:5 that were 1500 μm2. The hiPSC-CMs were harvested and plated on either stiff silanized glass or soft polyacrylamide (PAA) gels and analyzed with immunocytochemistry. Using specific shape constraints, cytoskeletal and myofibril structural organization were quantified to characterize the structural maturity of the hiPSC-CMs. Results demonstrate that with the addition of phenylephrine (PE), hiPSC-CMs attach to the patterned surface, fill the shape, and provide larger and more aligned sarcomeres. The specific patterns on a soft substrate elicit greater structural maturity, which can be seen three days after microcontact printing.
- Notes:
- Thesis (Sc. M.)--Brown University, 2018
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Citation
Paredes, Maria Guadalupe,
"Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine"
(2018).
Biomedical Engineering Theses and Dissertations.
Brown Digital Repository. Brown University Library.
https://doi.org/10.26300/9xj2-cd57
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Biomedical Engineering Theses and Dissertations
Theses and Dissertations for the Biomedical Engineering department....