- Title Information
- Title
- Author manuscript: "The stage-specific testicular germ cell apoptotic response to low-dose X-irradiation and 2,5-hexanedione combined exposure. I: Validation of the laser capture microdissection method for qRT-PCR array application"
- Abstract
- This is a post-print of the article allowed by the publisher to be deposited in the Brown Digital Repository. We optimized the LCM method optimized to generate RNA quantities sufficient for qRT-PCR array analysis without amplification, and were able to validate the method through direct comparison of results from unamplified and amplified RNA from individual samples. The addition of an amplification step for gene expression studies using LCM RNA resulted in a bias, especially for low abundance transcripts. Although the amplification bias was consistent across samples, researchers should use caution when comparing results generated from amplified and unamplified LCM RNA.
- Name
- Name Part
- Catlina, Natasha R.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name
- Name Part
- Husea, Susan M.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name
- Name Part
- Boekelheide, Kim
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Origin Information
- Date Created
- 2014
- Subject (Local)
- Topic
- laser capture microdissection
- Subject (Local)
- Topic
- LCM
- Subject (Local)
- Topic
- testicular germ cell
- Subject (Local)
- Topic
- apoptotic response
- Subject (Local)
- Topic
- PCR array
- Subject (Local)
- Topic
- qRT-PCR array
- Type of Resource
- text
- Genre
- research article
- Note:
funding
- This work received funding from the National Institute of Environmental Health Sciences grant P42ES013660Â