- Title Information
- Title
- Video from manuscript "A 3D fish liver model for aquatic toxicology: Morphological changes and Cyp1a induction in PLHC-1 microtissues after repeated benzo(a)pyrene exposures"
- Abstract
- Video file for manuscript "A 3D fish liver model for aquatic toxicology: Morphological changes and Cyp1a induction in PLHC-1 microtissues after repeated benzo(a)pyrene exposuresPMID: 28282620 and PMCID: PMC5436724. Version of Record is Published in Edited Form as: Aquatix Toxicology. 2017 May;186:134-144. doi:10.1016/j.aquatox.2017.02.018. This study characterizes a novel fish liver microtissue model, produced with the cell line PLHC-1, as an in vitro aquatic toxicity testing platform. These 3D microtissues remain viable and stable throughout the 8-day testing period and relative to 2D monolayers, show increased basal expression of the xenobiotic metabolizing enzyme cytochrome P450 1A (Cyp1a). Imaged with 1μm z-slices. Nuclei stained with Hoechst 33342. Nuclear staining of whole microtissues demonstrates that the dense packing of cells observed in sections is representative of the entire 3D spheroid.
- Name
- Name Part
- Rodd, April L.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
- Creator
- Name
- Name Part
- Messier, Norma J.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
- Creator
- Name
- Name Part
- Vaslet, Charles A.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
- Creator
- Name
- Name Part
- Kane, Agnes B.
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/cre")
- Creator
- Origin Information
- Date Created
- 2016
- Subject (Local)
- Topic
- animal alternatives
- Subject (Local)
- Topic
- cytochrome P450 1A
- Subject (Local)
- Topic
- Cyp1a
- Subject (Local)
- Topic
- fish
- Subject (Local)
- Topic
- hepatocytes
- Subject (Local)
- Topic
- morphology
- Subject (Local)
- Topic
- polycyclic aromatic hydrocarbons
- Subject (Local)
- Topic
- PAHs
- Type of Resource
- moving image
- Genre
- digital video
- Access Condition:
use and reproduction
(href="http://creativecommons.org/licenses/by/4.0/")
-
This work is licensed under a Creative Commons Attribution 4.0 International License
- Access Condition:
logo
(href="https://licensebuttons.net/l/by/4.0/88x31.png")
- Note:
funding
- This work was supported by NIEHS Superfund Research Program Grant P42 ES013660 and the generous support of Donna McGraw Weiss and Jason Weiss. A.L.R. is funded by NIEHS Training
Grant T32 ES007272 and the Institute at Brown for Environment and Society (IBES).
- Identifier:
DOI
- 10.26300/dypg-nh45