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Year:: 2017
Contributor:: Rodd, April L. (Author); Messier, Norma J. (Author); Vaslet, Charles A. (Author); Kane, Agnes B. (Author)
Subject:: animal alternatives; cytochrome P450 1A; Cyp1a; fish; hepatocytes; morphology; polycyclic aromatic hydrocarbons; PAHs

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Abstract:: This is a post-print manuscript of the article allowed by the publisher to be deposited in the Brown Digital Repository. This study characterizes a novel fish liver microtissue model, produced with the cell line PLHC-1, as an in vitro aquatic toxicity testing platform. These 3D microtissues remain viable and stable throughout the 8-day testing period and relative to 2D monolayers, show increased basal expression of the xenobiotic metabolizing enzyme cytochrome P450 1A (Cyp1a). This study demonstrates the capabilities of this PLHC-1 microtissue model for sensitive assessment of liver toxicants over time and following single and repeated exposures.
Notes:: This work was supported by NIEHS Superfund Research Program Grant P42 ES013660 and the generous support of Donna McGraw Weiss and Jason Weiss. A.L.R. is funded by NIEHS Training Grant T32 ES007272 and the Institute at Brown for Environment and Society (IBES).

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Rodd, April L., Messier, Norma J., Vaslet, Charles A., et al., "Author manuscript: 'A 3D fish liver model for aquatic toxicology: Morphological changes and Cyp1a induction in PLHC-1 microtissues after repeated benzo(a)pyrene exposures'" (2017). Brown Superfund Project 2: Adverse Human Health Impacts of Nanomaterials Digital Archive, Brown Superfund Presentations & Publications. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:799386/

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Brown Superfund Project 2: Adverse Human Health Impacts of Nanomaterials Digital Archive

This archive is for the long-term preservation and public dissemination of data and digital research products collected and created by researchers associated with Brown University Superfund Research Program Project 2: "Adverse Human Health Impacts of Nanomaterials". Project Leader: Agnes B. …
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Brown Superfund Presentations & Publications

This collection contains research articles, conference papers, research posters, and slide presentations associated with Brown University Superfund Research Program's investigators and research projects.
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Author manuscript: "A 3D fish liver model for aquatic toxicology: Morphological changes and Cyp1a induction in PLHC-1 microtissues after repeated benzo(a)pyrene exposures"