Visualizing Nanoparticles: Integration of Methods Used for Biological and Polymeric Interfaces

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Overview

Title
Visualizing Nanoparticles: Integration of Methods Used for Biological and Polymeric Interfaces
Contributors
Rajpara, Seeta (Creator)
Mathiowitz, Edith (Advisor)
Zielinski-Habershaw, Beth A. (Reader)
Marshall, John. (Reader)
Brown University. Department of Molecular Pharmacology, Physiology and Biotechnology (Sponsor)
Doi
10.7301/Z03T9FRX
Copyright Date
2016
Abstract
One of the major challenges in the field of controlled drug delivery systems involves overcoming the poor bioavailability of some therapeutic compounds. Polymeric microspheres can be used to deliver these drugs efficiently and safely. This novel method requires understanding the biodistribution of the microspheres within the tissues of interest. Previously, research has validated the presence of polystyrene microspheres in several organs; however, the methods required the tissues to be homogenized. The current study focuses on optimizing routine staining methods to account for polymeric microspheres within biological tissues. We found that adjusting some of the solvents used in routine staining procedures will allow for the preservation of hydrophobic polymer microspheres for further study and imaging. This could potentially give us more insight into the mechanisms of uptake after administration. The second focus of this study was to utilize these optimized procedures to analyze cell recruitment after delivery of growth factors and cytokines encapsulated in biodegradable polymeric microspheres. Our objective in this study was shifted from visualizing polymer microspheres to qualitatively assessing cell recruitment using histological methods. After optimizing the procedures necessary to visualize tissue morphology, we observed that the treatments with specific growth factors resulted in some recruitment of cells to the site of administration.
Keywords
drug delivery systems
Drugs--Controlled release
polymer chemistry
Brown adipose tissue
adipose tissues
polymeric nanospheres
cellular recruitment
Notes
Thesis (Sc. M.)--Brown University, 2016
Extent
111 p.

Citation

Rajpara, Seeta, "Visualizing Nanoparticles: Integration of Methods Used for Biological and Polymeric Interfaces" (2016). Molecular Pharmacology, Physiology, and Biotechnology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://doi.org/10.7301/Z03T9FRX

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