Alginate hydrogels for 3-D cell culture applications

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Title
Alginate hydrogels for 3-D cell culture applications
Contributors
Skipper, Thomas (creator)
Michelle Dawson (advisor)
Brown University. Karen T. Romer Undergraduate Teaching and Research Awards (research program)
Date Created
2017
Abstract
Cancer cell culture in three dimensions has become a research priority due to its closer representation of in vivo conditions. Understanding microenvironment interactions in a more realistic in vitro setting is an important aim of this project. Alginate is a naturally-occurring anionic, biocompatible, biodegradable polymer obtained from brown seaweed. By cross-linking 1% and 2% (w/v) alginate with CaCl2 in a controlled manner, we obtained homogeneous gels at a range of stiffness and porosity. Subsequent cell culture in sterile conditions allowed us to study their comparative morphology, motility, and viability in the gels, and to draw conclusions about the cells’ metastatic tendencies in different microenvironments.
Keywords
Alginate
cancer
Extent
1 poster

Citation

Skipper, Thomas, "Alginate hydrogels for 3-D cell culture applications" (2017). Summer Research Symposium. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:824492/

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