Impacts of genotoxic agents on rates of ribosomal mutations in the 30S ribosomal rspE gene.The RspE gene is responsible for encoding a portion of the 30S ribosomal subunit in B. subtilis. Single nucleotide mutations in the RspE gene provide protection from the effects of the ribosome targeting antibiotic spectinomycin. The goal of the project is to study the impacts of genotoxic agents on the mutations in the RspE gene. We will define whether specific treatments result in different mutation rates or a preference for specific nucleotide changes. Sequencing a library of these mutants using the Illumina next generation sequencing platform will allow to characterize the mutations and observe how various genotoxic agents impact mutation types and rates.Lee, StellacreatorPeter BelenkyadvisorBrown University. MMIBrown University. Karen T. Romer Undergraduate Teaching and Research Awardsresearch programDrug resistance in microorganismsEnglishstill imagepostersriuProvidence, RIBrown University20181 posterborn digital10.26300/fxsn-p449