- Title Information
- Title
- The role of LINE-1 retrotransposition in the accumulating DNA damage of neural stem cells
- Type of Resource
- text
- Name:
Personal
- Name Part
- Huang, Constance
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Kreiling, Jill
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Reenan, Robert
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Liu, Judy
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Department of Molecular Pharmacology, Physiology and Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2019
- Physical Description
- Extent
- 5, 23 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2019
- Genre (aat)
- theses
- Abstract
- Neural stem cells (NSCs) are multipotent cells that have the potential to differentiate into multiple lineages that ultimately populate the brain with a mosaic of cells of various function. However, in the later stages of life, the loss of proliferative capability of NSCs can lead to the onset of many neurodegenerative diseases. One of the possible mechanisms behind this loss of function could be the expression of LINE-1 (L1) retrotransposable elements in the brain. These self-autonomous elements have the capability of randomly inserting into the host genome and are found to be highly expressed in the brain as well as throughout various tissues in the body. There is mounting evidence to show age-related changes to chromatin structure results in the increased rates of unsuccessful retrotransposable events that can ultimately lead to accumulating DNA damage. With the presence of L1 found to be in the brain, it is possible that the brain is susceptible to increasing DNA damage that has been demonstrated to induce senescence in neural stem cells.
To provide insight into this possible mechanism of action, NSCs were transfected L1 elements under tetracycline inducible promoters. Once activated, the NSCs were screened for DNA damage and senescence associated phenotype (SASP) markers to determine its impact on the functionality of NSCs. This project aims to implicate the role of L1 expression in the accumulation of DNA damage that ultimately results in the senescence and loss of function in neural stem cells.
- Subject
- Topic
- Adult Neurogenesis
- Subject
- Topic
- retrotransposon
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00837570")
- Topic
- Brain
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01036275")
- Topic
- Neural stem cells
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20190603
- Identifier:
DOI
- 10.26300/zzp8-kf77
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.