- Title Information
- Title
- Characterization of Vegetal RNP Transport Granules in Xenopus laevis Oocytes.
- Name:
Personal
- Name Part
- Jeschonek, Samantha Page
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Mowry, Kimberly
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Creton, Robbert
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- DeLong, Alison
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Wessel, Gary
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Blower, Michael
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Department of Molecular Biology, Cell Biology and Biochemistry
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2018
- Physical Description
- Extent
- xvii, 215 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Ph. D.)--Brown University, 2018
- Genre (aat)
- theses
- Abstract
- Subcellular mRNA localization is a conserved mechanism across eukaryotes, which is found in both somatic and germ cells. RNA localization in
combination with local translation provides a means to generate polarized protein synthesis. In Xenopus laevis oocytes, vg1 mRNA, which encodes a TGFβ growth factor family member, is localized to the vegetal cortex of developing oocytes. Vegetal RNA localization ensures that only the vegetal most blastomeres inherit vg1 mRNA, resulting in proper germ layer patterning. Vg1 mRNA is actively transported as a ribonucleoprotein (RNP) complex, but only a handful of vg1 RNP components are as yet known. Understanding the composition of transport RNPs is critical to reveal not only their molecular nature, assembly, and biochemical properties, but also may provide links to cytoplasmic RNPs in other systems. To that end, we have developed a multi-step approach to biochemically isolate endogenous vegetal transport granules. Proteomic analysis of the transport granules revealed both protein constituents found in other known cytoplasmic RNP granules—including stress granules, processing bodies, neuronal granules and germ granules—as well as a set of unique proteins. Preliminary transcriptome
analysis further indicates that vegetal transport granules contain heterotypic populations of RNAs, also consistent with some cytoplasmic granule types. Together our results suggest that vegetal transport RNPs represent a new class of cytoplasmic RNP granules.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01182017")
- Topic
- Xenopus laevis
- Subject
- Topic
- RNP granules
- Subject
- Topic
- RNA localization
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01049494")
- Topic
- Ovum
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20190603
- Identifier:
DOI
- 10.26300/m1mg-nk10
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Access Condition:
restriction on access
- Collection is open for research.
- Type of Resource (primo)
- dissertations