- Title Information
- Title
- Supplementary data from "Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells"
- Abstract
- An automated counting method was developed in ImageJ to quantify MNGs in digital images of hematoxylin-stained rat fetal testis tissue sections. Timed pregnant Sprague Dawley rats were exposed by daily oral gavage from gestation day 17 to 21 with one of eight phthalate test compounds or corn oil vehicle. Both the manual counting method and the automated image analysis method identified di-n-butyl phthalate, butyl benzyl phthalate, dipentyl phthalate, and di-(2-ethylhexyl) phthalate as positive for induction of MNGs. Dimethyl phthalate, diethyl phthalate, the brominated phthalate di-(2-ethylhexyl) tetrabromophthalate, and dioctyl terephthalate were negative. The correlation between automated and manual scoring metrics was high (r = 0.923). Results of MNG analysis were consistent with these compounds’ anti-androgenic activities, which were confirmed in an ex vivo testosterone production assay. In conclusion, we have developed a reliable image analysis method that can be used to facilitate dose-response studies for the reproducible induction of MNGs by in utero phthalate exposure.
- Genre
- supplementary material
- Name:
Personal
- Name Part
- Spade, Daniel J.
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Yue Bai, Cathy
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Lambright, Christy
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Conley, Justin M.
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Boekelheidea, Kim
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Gray, L. Earl
- Role
- Role Term:
Text
- creator
- Origin Information
- Date Created
(keyDate="yes", encoding="w3cdtf")
- 2018
- Type of Resource
- multimedia
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01020062")
- Topic
- Microscopy
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01148269")
- Topic
- Testis
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01090300")
- Topic
- Rats
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00881354")
- Topic
- Counting
- Language
- Language Term:
Text (ISO639-2B)
- English
- Note:
funding
- This research is supported by the National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program P42ES013660
- Access Condition:
use and reproduction
(href="https://creativecommons.org/licenses/by/4.0/legalcode")
- Attribution 4.0 International (CC BY 4.0)
- Access Condition:
logo
(href="https://licensebuttons.net/l/by/4.0/88x31.png")
- Identifier:
DOI
- 10.26300/hmt5-kx75