- Title Information
- Title
- Developing Methods for Perinatal Health Screening
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Piserchia, Jennifer
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Tripathi, Anubhav
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Wessel, Gary
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Raimondo, Theresa
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biomedical Engineering
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2024
- Physical Description
- Extent
- 9, 45 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2024
- Genre (aat)
- theses
- Abstract
- Screening for maternal and prenatal health conditions allows for better patient care and improved health outcomes. In maternal health, hypertensive conditions like preeclampsia, which leads to an increased risk for a number of fatal complications, lack specific and sensitive screening tools. In fetal health, chromosomal abnormalities are a primary concern during development, but standard diagnostic test methods carry a risk of miscarriage, therefore there is a need for a noninvasive screening method for aneuploidy. In response to the need for new screening tools for preeclampsia and aneuploidy, two perinatal screening methods were developed: a dual-bead based assay to screen for preeclampsia and an automated capillary electropherogram based assay to screen for aneuploidy. The dual-bead assay relies on an antibody-carbohydrate sandwich used to capture sFlt-1, a protein biomarker for preeclampsia, for detection using qPCR. The aneuploidy screening method uses modified cfDNA and probes to bind DNA fragments for a non-quantitative, size based detection of aneuploidy. Experiments were unable to be executed for the preeclampsia study, but expected results are detailed. For the aneuploidy study, a series of experiments were performed to optimize a double DNA hybridization using model cfDNA and DNA probes. Results showed that preparing samples with a pH of 6 is optimal for hybridization. Future work should be done at a higher sample size to verify these results. A novel method to screen for aneuploidy in a non-invasive and non-quantitative way is presented.
- Subject
- Topic
- Perinatal Health
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01075044")
- Topic
- Preeclampsia
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00831950")
- Topic
- Biochemical markers
- Subject
- Topic
- diagnostics
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00808717")
- Topic
- Aneuploidy
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00940094")
- Topic
- Genetic screening
- Subject
- Topic
- NIPT
- Subject
- Topic
- NIPS
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00892326")
- Topic
- Diagnosis, Noninvasive
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00832194")
- Topic
- Biological assay
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20240502