Title Information
Title
Quantifying Microenvironmental Forces in Engineered Tissue Models
Type of Resource (primo)
dissertations
Name: Personal
Name Part
Chordiya, Prerna
Role
Role Term: Text
creator
Name: Personal
Name Part
Kesari, Haneesh
Role
Role Term: Text
Reader
Name: Personal
Name Part
Dawson, Michelle
Role
Role Term: Text
Reader
Name: Personal
Name Part
Darling, Eric
Role
Role Term: Text
Advisor
Name: Corporate
Name Part
Brown University. Biology and Medicine: Biomedical Engineering
Role
Role Term: Text
sponsor
Origin Information
Copyright Date
2026
Physical Description
Extent
xiv, 48 p.
digitalOrigin
born digital
Note: thesis
Thesis (Sc. M.)--Brown University, 2026
Genre (aat)
theses
Abstract
Abstract of Microenvironmental Forces in Engineered Tissue Models, Prerna Chordiya, ScM, Brown University, May 2026 Microenvironmental mechanical forces are key regulators of cellular behavior, yet their origin and modulation within three-dimensional (3D) systems remain incompletely understood. In particular, the respective roles of cell-cell interactions and extracellular matrix (ECM) composition in governing these forces are not well defined. To address this, hypercompliant microparticles (HCMPs) were used to quantify microenvironmental mechanical parameters in two different models. First, the dog bone system to investigate microenvironmental forces in early musculoskeletal regeneration. And second, a cartilage spheroid model to evaluate changes in forces following enzymatic matrix degradation. Mechanical parameters such as pressure, volume, and elastic energy were analyzed to capture both system-level and sensor-level responses. The results indicate that while cellular interactions contribute to first-generation force production as seen in the dog bone model, the ECM is essential for stabilizing and modulating the mechanical microenvironment. Matrix degradation leads to measurable alterations in microenvironmental mechanical states, highlighting the role of ECM composition in regulating distribution. Together, this study provides a framework for directly measuring and interpreting microenvironmental forces in 3D systems, with implications for the disease modeling and tissue engineering.
Subject
Topic
engineered tissue
Subject
Topic
Microtissues
Subject
Topic
3D tissue engineering
Subject
Topic
cartilage engineering
Subject
Topic
musculokeletal tissues
Language
Language Term (ISO639-2B)
English
Record Information
Record Content Source (marcorg)
RPB
Record Creation Date (encoding="iso8601")
20260516