- Title Information
- Title
- Pseudotyping Lentivirus in 293T cells to Measure Antibodies that Block Entry of Respiratory Syncytial Virus
- Type of Resource (primo)
- dissertations
- Name:
Personal
- Name Part
- Parakh, Harshini
- Role
- Role Term:
Text
- creator
- Name:
Personal
- Name Part
- Jamieson, Amanda
- Role
- Role Term:
Text
- Reader
- Name:
Personal
- Name Part
- Nau, Gerard
- Role
- Role Term:
Text
- Advisor
- Name:
Personal
- Name Part
- Tripathi, Anubhav
- Role
- Role Term:
Text
- Reader
- Name:
Corporate
- Name Part
- Brown University. Biology and Medicine: Biotechnology
- Role
- Role Term:
Text
- sponsor
- Origin Information
- Copyright Date
- 2025
- Physical Description
- Extent
- , 42 p.
- digitalOrigin
- born digital
- Note:
thesis
- Thesis (Sc. M.)--Brown University, 2025
- Genre (aat)
- theses
- Abstract
- Respiratory syncytial virus (RSV) is a significant cause of respiratory illness in the elderly, necessitating effective vaccines and reliable methods to measure immune responses. This study aimed to measure vaccine-induced neutralizing antibody responses to RSV using a pseudotyped lentivirus system in a high-risk elderly cohort. Pre- and post-vaccination serum samples from nursing home residents were tested using a pseudotyped lentiviral vector generated in 293T cells expressing the RSV fusion (F) long protein. Neutralization assays were conducted to measure functional antibody activity against RSV, while enzyme-linked immunosorbent assays (ELISA) quantified total RSV-specific antibodies. Post-vaccination samples showed a significant rise in neutralizing titers against the RSV F protein from both the A2 and B strains of the virus, indicating that vaccination elicited robust F-specific neutralizing antibodies across subtypes. In contrast, no significant increase in antibodies against the RSV attachment (G) glycoprotein was observed, reflecting the fact that the vaccine is comprised only of F protein. ELISA results confirmed an overall increase in RSV-specific antibody levels after vaccination and were modestly correlated with neutralization titers, suggesting that not all binding antibodies had neutralizing capability. These findings demonstrate that the 293T cell-based pseudotyped lentivirus neutralization assay is a potential tool for evaluating RSV vaccine-induced immunity. The F-specific neutralizing antibody response supports the RSV F protein as a promising target for vaccines, particularly to protect high-risk elderly populations such as nursing home residents.
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/00967867")
- Topic
- Immune response
- Subject (fast)
(authorityURI="http://id.worldcat.org/fast", valueURI="http://id.worldcat.org/fast/01095811")
- Topic
- Respiratory syncytial virus
- Subject
- Topic
- Pseudotyping
- Subject
- Topic
- Clinical serum samples
- Language
- Language Term (ISO639-2B)
- English
- Record Information
- Record Content Source (marcorg)
- RPB
- Record Creation Date
(encoding="iso8601")
- 20250707