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Neutrophil Phagocytosis of P. aeruginosa and S. aureus as a basis for CRISPR-Cas9 screening

Description

Abstract:
Neutrophils are myeloid derived leukocytes that play an essential role in the innate immune system. They are often referred to as “first responders” to sites of inflammation, and are activated within minutes of trauma or an infection. They are capable of extracting themselves from circulation to enter tissues where they can kill invasive pathogens by engulfing and internalizing them, a process known as phagocytosis. With the rise of drug resistant bacteria strains such as Pseudomonas aeruginosa and Staphylococcus aureus, understanding the process of how neutrophils phagocytize these pathogens is more important than ever. We found that progenitor-­‐derived neutrophils effiiently internalize S. aureus, but not P. aeruginosa, and do so in an actin-­‐dependent manner. This study attempts to assess the genetic mechanisms responsible for neutrophil phagocytosis of bacteria in order to identify potential targets for modulating the phagocytic capacity of neutrophils and potentially enhance their ability to eradicate pathogens. Further findings of this study may help develop therapies to promote this aspect of innate immunity to battle drug resistant infections.

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Citation

Odzer, Jamie, Alamanzar, Rafael, and Lefort, Craig T., "Neutrophil Phagocytosis of P. aeruginosa and S. aureus as a basis for CRISPR-Cas9 screening" (2017). Warren Alpert Medical School Academic Symposium. Brown Digital Repository. Brown University Library. https://doi.org/10.26300/ma0s-mr10

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Collection:

  • Warren Alpert Medical School Academic Symposium

    The Warren Alpert Medical School Academic Symposium is an annual event at Warren Alpert Medical School of Brown University that provides Year II medical students a venue to present their summer research in a poster format. Participation in the Symposium …
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