- Title Information
- Title
- Elucidating the Determinants of Chemotherapy Response: A Single-Cell Transcriptomic Characterization of Chemosensitive and Resistant Tumors from Triple-Negative Breast Cancer Patients
- Abstract
- Triple-negative breast cancer (TNBC) drives disproportionate mortality, yet only ~50% of patients achieve pathological complete response to neoadjuvant chemotherapy and tumor-intrinsic determinants of resistance remain undefined. We performed single-cell single-cell transcriptomic profiling of grade 3 TNBC core-needle biopsies), comparing chemotherapy-sensitive (n=4) and chemotherapy-resistant (n=4) tumors. InferCNV confirmed malignant identity and revealed broad genomic alterations; differential expression and pathway enrichment identified distinct tumor-intrinsic states separating resistant from sensitive tumors, while spatial analysis localized resistant states within the tumor ecosystem. These findings establish a framework for stratifying TNBC by tumor-intrinsic molecular vulnerability rather than histopathologic subtype alone.
- Name:
Personal
- Name Part
- Justin M. Kim
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Marygrace C. Trousdell
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Claire Gould
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Matthew Manwaring
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Yihong Wang
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Ying Ma
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Aditi Marehalli
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Nick Kuburich
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Patrick Schupp
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Petra den Hollander
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Name:
Personal
- Name Part
- Sendurai A. Mani
- Role
- Role Term (marcrelator)
(authorityURI="http://id.loc.gov/vocabulary/relators", valueURI="http://id.loc.gov/vocabulary/relators/aut")
- Author
- Subject
- Topic
- Breast cancer
- Subject
- Topic
- Triple-negative breast cancer
- Subject
- Topic
- Single-cell
- Subject
- Topic
- Chemotherapy resistance
- Subject
- Topic
- Neoadjuvant chemotherapy
- Subject
- Topic
- Tumor heterogeneity
- Subject
- Topic
- Spatial transcriptomics
- Access Condition:
use and reproduction
- All rights reserved
- Access Condition:
rights statement
(href="http://rightsstatements.org/vocab/InC/1.0/")
- In Copyright
- Type of Resource (primo)
- text_resources
- Genre (aat)
(valueURI="http://vocab.getty.edu/aat/300426530")
- instructional posters
- Origin Information
- Place
- Place Term:
Code (MARC Country Code)
(authorityURI="http://www.loc.gov/marc/countries/")
- riu
- Place Term:
Text
- Providence, Rhode Island
- Publisher (naf)
(authorityURI="http://id.loc.gov/authorities/names")
- Brown University Library
- Date Created
(keyDate="yes", encoding="w3cdtf")
- 2026
- Date Issued
(encoding="w3cdtf")
- 2026-06-04
- Physical Description
- Extent
- 1 document
- digitalOrigin
- born digital
- Identifier:
DOI
- 10.26300/r4rt-7171