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<mods:titleInfo>
<mods:title>Elucidating the Determinants of Chemotherapy Response: A Single-Cell Transcriptomic Characterization of Chemosensitive and Resistant Tumors from Triple-Negative Breast Cancer Patients</mods:title>
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<mods:abstract>Triple-negative breast cancer (TNBC) drives disproportionate mortality, yet only ~50% of patients achieve pathological complete response to neoadjuvant chemotherapy and tumor-intrinsic determinants of resistance remain undefined. We performed single-cell single-cell transcriptomic profiling of grade 3 TNBC core-needle biopsies), comparing chemotherapy-sensitive (n=4) and chemotherapy-resistant (n=4) tumors. InferCNV confirmed malignant identity and revealed broad genomic alterations; differential expression and pathway enrichment identified distinct tumor-intrinsic states separating resistant from sensitive tumors, while spatial analysis localized resistant states within the tumor ecosystem. These findings establish a framework for stratifying TNBC by tumor-intrinsic molecular vulnerability rather than histopathologic subtype alone.</mods:abstract>
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<mods:name type="personal">
<mods:namePart>Justin M. Kim</mods:namePart>
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<mods:name type="personal">
<mods:namePart>Marygrace C. Trousdell</mods:namePart>
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<mods:name type="personal">
<mods:namePart>Claire Gould</mods:namePart>
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<mods:name type="personal">
<mods:namePart>Matthew Manwaring</mods:namePart>
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<mods:name type="personal">
<mods:namePart>Yihong Wang</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Ying Ma</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Aditi Marehalli</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Nick Kuburich</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Patrick Schupp</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Petra den Hollander</mods:namePart>
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<!-- author -->
<mods:name type="personal">
<mods:namePart>Sendurai A. Mani</mods:namePart>
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<mods:subject>
<mods:topic>Breast cancer</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Triple-negative breast cancer</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Single-cell</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Chemotherapy resistance</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Neoadjuvant chemotherapy</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Tumor heterogeneity</mods:topic>
</mods:subject>
<mods:subject>
<mods:topic>Spatial transcriptomics</mods:topic>
</mods:subject>
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<mods:accessCondition type="use and reproduction">All rights reserved</mods:accessCondition>
<mods:accessCondition type="rights statement" xlink:href="http://rightsstatements.org/vocab/InC/1.0/">In Copyright</mods:accessCondition>
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<mods:placeTerm type="code" authority="marccountry" authorityURI="http://www.loc.gov/marc/countries/">riu</mods:placeTerm>
<mods:placeTerm type="text">Providence, Rhode Island</mods:placeTerm>
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<mods:publisher authority="naf" authorityURI="http://id.loc.gov/authorities/names">Brown University Library</mods:publisher>
<mods:dateCreated keyDate="yes" encoding="w3cdtf">2026</mods:dateCreated>
<mods:dateIssued encoding="w3cdtf">2026-06-04</mods:dateIssued>
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<mods:extent>1 document</mods:extent>
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<mods:identifier type="doi">10.26300/r4rt-7171</mods:identifier></mods:mods>