The Effect of Matrix Metalloproteinase Inhibitors on Three-Dimensional Dermal Fibroblast Tissue Rings

Padgett, Becka

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Overview

Year:: 2021
Contributor:: Padgett, Becka (creator); Morgan, Jeffrey (Advisor); Shukla, Anita (Reader); Ip, Blanche (Reader); Brown University. Biology and Medicine: Biomedical Engineering (sponsor)
Genre:: theses
Subject:: Matrixmetalloproteinase; Microtissue Manipulation
Extent:: vi, 34 p.

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Description

Abstract:: In this research, two inhibitors of MMPs were added to dermal fibroblast tissue rings that were allowed to self-assemble and produce their own ECM. One day after seeding, the media was replaced with media supplemented with incyclinide or doxycycline hyclate, or vehicle control media. Brightfield images of the rings were taken on days 1, 2, 5, 8, and 15 after seeding. Three rings per treatment group were also fixed on days 5, 8, and 15. These fixed rings were later imaged with OCT by Karl Anderson. With the OCT we were able to get a cross-sectional view of the tissues, allowing us to measure ring height, width, and cross-sectional area. Incyclinide, an inhibitor of MMP-2 and MMP-14 showed the stronger effect, with the rings growing in thickness, height, and cross-sectional area and a roughness at the surface of the rings was also seen. We didn’t see this roughness when the rings were treated with doxycycline hyclate, an inhibitor of collagenases, but there was a small increase in ring thickness.
Notes:: Thesis (Sc. M.)--Brown University, 2021

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Citation

Padgett, Becka, "The Effect of Matrix Metalloproteinase Inhibitors on Three-Dimensional Dermal Fibroblast Tissue Rings" (2021). Biomedical Engineering Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:rxc5nvua/

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Biomedical Engineering Theses and Dissertations

Theses and Dissertations for the Biomedical Engineering department.
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